Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-01-28
1994-12-27
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435259, C12Q 168, C12P 1934, C12N 106
Patent
active
053765273
ABSTRACT:
The invention provides a rapid process for lysing Mycobacteria. In one embodiment is provided a process for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of heat. The process of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.
REFERENCES:
A. Brisson-Noel et al., The Lancet Nov. 4, p. 1069 (1989).
U. Stritharan et al., Molecular and Cellular Probes 5:385 (1991).
Saiki, R. (1988) Science, vol. 239, "Primer Directed Enzymatic Amplification of DNA w/a Thermostable DNA Polymerase". pp. 487-491.
Boddinghaus, B. et al. (Aug. 1990) Journal of Clinical Microbiology, vol. 28(8), pp. 1751-1759.
Cormican et al., "Use of polymerase chain reaction for early identification of Mycobacterium tuberculosis in positive cultures", pp. 601-604, Jul. 1992, London GB, Journal Clinical Pathology vol. 45(7).
Cormican et al., "Rapid identification of cultured Mycobacterium tuberculosis by specific primer directed DNA amplification", abstract, Nov. 1991, Edinburgh Scotland, J. Medical Microbiol, vol. 35(5).
Down James A.
Howard Adriann J.
Keating William E.
Robson Jillian A.
Arthur Lisa
Becton Dickinson and Company
Highet David W.
Parr Margaret
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