Process for determination of glycosylated hemoglobin and reagent

Details Process for determination of glycosylated hemoglobin and reagent Process for determination of glycosylated hemoglobin and reagent

1982-02-19

1983-08-16

Turk, Arnold

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

Glycosylated hemoglobin

436175, G01N 3366, G01N 3372

Patent

active

043992277

ABSTRACT:
An improved process for determining glycosylated hemoglobin wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA.sub.1 value is substantially eliminated by reacting the hemolysate with a primary amine and a hydrazine compound and thereafter subjecting the hemolysate to chromatography. The process enables reliable evaluation of the HbA.sub.1 value as a long-term parameter in diabetic metabolism control.

REFERENCES:
Nathan et al., Diabetes, 30:700-701, Aug. 1981.
Innanen et al., Clinical Chemistry, vol. 27, No. 8, 1981, pp. 1478-1479.
Nathan, Clinical Chemistry, vol. 27, No. 7, 1981, pp. 1261-1263.

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