Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1984-12-13
1988-08-23
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 32, 435 33, C12Q 170, C12Q 118, C12Q 120
Patent
active
047660635
ABSTRACT:
The presence of fermentation inhibiting activity in a sample is determined by contacting the sample with bacterial strains and detecting the inhibition of the activity of the strains. A carrier (1) carrying microtitration cups arranged in four rows of cups (8) is provided. The cups of the same row contain a lyophilisate comprising a given bacterial strain. Each sample to be tested, effected in a tube (3), is diluted in a tube (4) containing a culture medium and bromocresol purple and then poured into the cups of a given column by means of an automatic pipette and cones (5). After incubation, a drop of janus green reagent in tube (6) is introduced in each cup and, after a new incubation, the number of cups which have produced a change in color indicates the degree of contamination of the mixture. The process may be applied in the determination of bacteriophages present in cheese-making vats.
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Staining Procedures, G. Clark (editor), Fourth Edition, Williams & Wilkins, Baltimore, 1981, pp. 275-276, 281-282, 446-447.
Chemical Abstracts, vol. 89, 1978, Abstract No. 20104c, Kruszewska et al.
Biological Abstracts, vol. 71, 1981, Abstract No. 29531.
Deck Randall E.
l'Air Liquide, Societe Anonyme Pour l'Etude et l'Exploitation De
Warden Robert J.
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