Printing oligonucleotide arrays using deprotection agents solely

Details Printing oligonucleotide arrays using deprotection agents solely Printing oligonucleotide arrays using deprotection agents solely

1996-04-19

1998-11-03

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 254, 536 2531, 536 2532, 536 2533, 530333, 530334, 530335, 435 911, 436518, C07H 2100, C07H 2102, C07K 500, C07K 1600

Patent

active

058310703

ABSTRACT:
A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a barrier material, a monomer sequence, a nucleoside, a deprotection agent, a carrier material, among other materials. The method and apparatus also relies upon standard DMT based chemistry, and a vapor phase deprotection agent such as solid TCA and the like.

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Boal et al, "Cleavage of oligodeoxyribonucleotides from controlled pore glass supports and their rapid deprotection by gaseous amines", Nucleic Acids Res. 24(15):3115-3117,1996.

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