Preparation of soluble chromogenic substrates

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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435 18, 435 22, 536 45, 536 56, 536117, 536118, 536120, C08B 3700

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043213642

ABSTRACT:
A chromogenic substrate for the assay of polysaccharide endo-hydrolases is the reaction product of a polysaccharide susceptible to endo-hydrolase degradation, a chemical reagent for increasing the solubility of the polysaccharide so as to increase its susceptibility to enzyme degradation, and a dye substance for coloring the polysaccharide and rendering it capable of estimation by absorption spectrometry. The amount of chemical reagent should be such as to produce a degree of chemical substitution in the range 0.06 to 0.6 DS.

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