Polynucleotides and polypeptides coded by said...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

Reexamination Certificate

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C435S252330, C435S252350, C435S228000, C435S320100, C435S252300, C435S325000, C536S023200

Reexamination Certificate

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07112425

ABSTRACT:
The invention concerns novel isolated natural or synthetic polynucleotides and polypeptides coded by said polynucleotides, involved in the synthesis of diketopiperazine derivatives, vectors comprising said polynucleotides, micro-organisms transformed with said polynucleotides, uses of said polynucleotides and said polypeptides, as well as methods for the synthesis of diketopiperazine derivatives, including cyclodipeptides and diketopiperazine derivatives 3- and 6- substituted by α,β-unsaturated amino acid side chains.

REFERENCES:
GenBank Accession No. AY129235,Streptomyces nourseiputative tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase gene, partial cds; albonoursin biosynthetic gene cluster, complete sequence; and putative NADP-specific glutamate dehydrogenase gene.
Gondry, M. et al. “Cyclic dipeptide oxidase fromStreptomyces nourseiIoslation, purification and partial characterization of a novel, amino acyl α, β-dehydrogenase”,Eur. J. Biochem., vol. 268 pp. 1712-1721, 2001.
Kanzaki, H. et al. “Enzymatic Conversion of Cyclic Dipeptides to Dehydro Derivatives that Inhibit Cell Division”,Journal of Bioscience and Bioengineering, vol. 90(1) pp. 86-89, 2000.
Kanzaki, H. et al. “Biosynthetic Intermediates of the Tetradehydro Cyclic Dipeptide Albonoursin Produced byStreptomyces albulusKO-23”,The Journal of Antibiotics, vol. 53 (11) pp. 1257-1264, 2000.
Lautru, S. et al. “The Albonoursin Gene Cluster ofS. noursei: Biosynthesis of Diketopiperazine Metabolites Independent of Nonribosomal Peptide Synthetases”,Chemistry&Biology, vol. 9 pp. 1355-1364, 2002.

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