Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1994-07-18
1997-07-08
Chereskin, Che S.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
4352523, 4353201, 435365, 435419, 536 236, 800205, C12N 121, C12N 500, C12N 514, C12N 506
Patent
active
056460295
ABSTRACT:
This invention provides plant arabinogalactan proteins (AGPs) and their genes. AGPs were isolated from Nicotiana alata, Nicotiana plumbaginafolia, and Pyrus communis. Amino acid sequences of isolated AGP peptide fragments are presented. Isolated AGP fragments were used to synthesize oligonucleotide probes to prepare oligonucleotide primers for PCR or prepare RNA probes to screen cDNA libraries of N. alata, N. plumbaginafolia, and P. communis. cDNA clones encoding amino acid sequences of isolated AGP fragments were isolated. The invention presents for the first time an intact AGP amino acid sequence derived from a corresponding AGP gene. The instant invention further provides methods useful in obtaining AGP genes encoding an AGP peptide comprising a specific isolated hydroxyproline-rich (OAST-rich) sequence or a specific isolated hydroxyproline-poor sequence.
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Bacic Antony
Chen Chao-Guang
Clarke Adrienne E.
Du He
Gane Alison M.
Chereskin Che S.
Cooperative Research Centre For Industrial Plant Biopolymers
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