Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-06-02
1998-03-03
Robinson, Douglas W.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
426607, 4351723, 4353201, 435419, 536 232, 536 236, 800205, 800255, 800DIG9, 800DIG17, 800DIG59, 800DIG65, 800DIG69, A01H 500, A01H 1510, C12N 514, C12N 1529, C12N 1552, C12N 1582
Patent
active
057237611
ABSTRACT:
This invention relates to plant thioesterases, specifically plant acyl-ACP thioesterases having substantial activity on palmitoyl-ACP substrates. DNA constructs useful for the expression of a plant palmitoyl-ACP thioesterase in a plant seed cell are described. Such constructs will contain a DNA sequence encoding the plant palmitoyl-ACP thioesterase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant palmitoyl-ACP thioesterase in seed tissue, as compared with other plant tissues, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant palmitoyl-ACP thioesterase for the modification of the proportion of free fatty acids produced in a plant seed cell. Plant palmitoyl-ACP thioesterase sequences exemplified herein include Cuphea, leek, mango and elm. Transgenic plants having increased levels of C16:0 fatty acids in their seeds as the result of expression of these palmitoyl-ACP thioesterase sequences are also provided.
REFERENCES:
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Kridl, et al. "Progress in Expression of Genes Controlling Fatty Acid Biosynthesis to Alter Oil Composition and Content in Transgenic Rapeseed" CRC Press, Control of Gene Expression Chapter 30 (1993): 481-498.
Davies, et al. "Developmental Induction, Purification and Further Characterization of 12:0-ACP Thioesterase from Immature Cotyledons of Umbelluria californica" Archives of Biochemistry and Biophysics (1991) 290: No. 1, Oct.: 37-45.
Knutzon, et al. "Modification of Brassica Seed Oil by Antisense Expression of a Stearoyl-Acyl Protein Desaturase Gene" Plant Biology (1992) 89: 2624-2628.
Voelker, et al. "Fatty Acid Biosynthesis Redirected to Medium Chains in Transgenic Oilseed Plants" Science (1992) 257: 72-74.
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Knauf, et al., "The Application of Genetic Engineering to Oilseed Crops", Trends in Biotechnology vol. 5, No. 2, Feb. 1987.
Persson, et al., "High Palimitic Acid Content in Summer Turnip Rape (Brassica campestris var annua L.)" Database CAB AN 86:25219 (from Cruciferae Newsletter No. 10, p. 137, (1985).
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Hawkins Deborah
Jones Aubrey
Kridl Jean
Voelker Toni A.
Yuan Ling
Calgene Inc.
Nelson Amy J.
Robinson Douglas W.
Scherer Donna E.
Schwedler Carl J.
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