Phytases, nucleic acids encoding them and methods of making...

Details Phytases, nucleic acids encoding them and methods of making... Phytases, nucleic acids encoding them and methods of making...

2004-09-01

2008-10-07

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S174000, C435S320100, C435S252300, C435S325000, C435S069100, C435S019000, C424S094600, C530S350000, C536S023200

Reexamination Certificate

active

07432097

ABSTRACT:
In one aspect, the invention provides a purified and modified phytase enzyme fromEscherichia coliK12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

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