Phytases and methods for making and using them

Details Phytases and methods for making and using them Phytases and methods for making and using them

2005-02-11

2008-10-07

Ramirez, Delia M (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S069700, C435S174000, C435S018000, C424S094600, C426S656000, C530S350000, C536S023200

Reexamination Certificate

active

07432098

ABSTRACT:
The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA ofE. coli.The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to aid in the digestion of phytate where desired. In particular, the phytases of the invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients. The phytases of the invention can be thermotolerant and/or thermostable. Also provided are methods for obtaining a variant polynucleotide encoding a phytase and for obtaining a phytase with thermostability or thermotolerant at high or low temperatures.

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