Food or edible material: processes – compositions – and products – Products per se – or processes of preparing or treating... – Plant material is basic ingredient other than extract,...
Reexamination Certificate
2004-03-25
2008-10-28
Prouty, Rebecca E. (Department: 1652)
Food or edible material: processes, compositions, and products
Products per se, or processes of preparing or treating...
Plant material is basic ingredient other than extract,...
C426S063000, C435S021000, C435S069100, C435S091100, C435S196000, C536S023100, C536S023200
Reexamination Certificate
active
07442398
ABSTRACT:
The present invention relates to phytase enzyme, a gene encoding the enzyme, and aCitrobactersp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced fromCitrobactersp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55° C., (d) a substrates phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) Michaelis constant of 0.3-0.5 mM utilizing phytate as substrate, and (f) high resistance to protease such as pepsin, trypsin, papain, elastase or pancreatin. The present invention also relates to the gene coding the phytase enzyme and theCitrobacter braakiiproducing the enzyme. The phytase enzyme and theCitrobacter braakiiproducing the enzyme of the present invention can be used in manufacturing a feed of monogastrics as feed additive and in recovering a specific decomposition product of phytate at low price.
REFERENCES:
patent: 6235517 (2001-05-01), Chu et al.
patent: 0779037 (1997-06-01), None
Kim et al., Isolation and characterization of a phytase with improved properties fromCitrobacter braakii. Biotechnol. Letters., 2003, vol. 25: 1231-1234.
Kim et al., Purification and characterization of a novel phytase fromCitrobacter brakiiYH-15 strain. 9thInternational Symposium on the Genetics of Industrial Microorganisms. Abstract P21-31, Jul. 1-5, Gyeonju, Korea (In IDS).
Lei, X.G, & Stahl, C.H., “Biotechnological development of effective phytases for mineral nutrition and environmental protection,” Appl Microbiol Biotechnol (2001) 57:474-481.
Kim, H.W, et al., “Isolation and characterization of a phytase with improved properties fromCitrobacter braakii,”Biotechnol. Lett., Aug. 2003, 25:1231-1234.
Kim, Young-Ok et al., “Purification and characterization of a novel phytase fromCitrobacter braakiiYIL-15” Abstract P21-31 from 9th International Symposium on the Genetics of Industrial Microorganisms, Jul. 2002.
Ullah, Abul H.J., et al., “Characterization of recombinant fungal phytase (phyA) expressed in tobacco leaves,”Biochemical and Biophysical Research Communications (1999) 264(1):202-206.
Kim Han Woo
Kim Kyung Kil
Kim Young Ok
Kong In Soo
Lee Jeong Ho
JHK Law
Kim Joseph Hyosuk
Prouty Rebecca E.
Raghu Ganapathirama
Republic of National Fisheries Research and Development Institut
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