Phenylalanyl tRNA synthetase from streptococcus pneumoniae

Details Phenylalanyl tRNA synthetase from streptococcus pneumoniae Phenylalanyl tRNA synthetase from streptococcus pneumoniae

1998-09-24

2000-12-19

Achutamurthy, Ponnathapu

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

C12N 900

Patent

active

061626252

ABSTRACT:
The invention provides phenylalanyl tRNA synthelase, pheS, (alpha) polypeptides and DNA (RNA) encoding pheS (alpha) polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pheS (alpha) polypeptides to screen for antibacterial compounds.

REFERENCES:
R. Calendar et al. "Purification and Physical Characterization of Tyrosyl Ribonucleic Acid Synthetases from Escerichia coli and Bacillus subtilis", Biochemistry, 5(5) p. 1681-1690 (1966).
J. Hughes et al., "How Does Pseudomonas Fluorescens, the Producing Organism of the Antibiotic Pseudomonic Acid A, Avoid Suicide?", FEBS Letters, 122(2) p. 322-324 (1980).
Brakhage et al., "Structure and Nucleotide Sequence of the Bacillus subtilis Phenylalanyl-tRNA Synthetase Genes," Biochime, 72 p. 725-734 (1990).
R. Kreutzer et al., "Structure of the Phenylalanyl-tRNA Synthetase Genes from Thermus thermophilus HB8 and Their Expression in E. coli," Nucleic Acids Research, 20(1) p. 4173-4178 (1992).
Fleischmann et al., "Whole-Genome Random Sequencing and Assembly of Haemophilus influenza Rd," Science 269 p. 496-512 (1995).
Brakhage et al. GenBank Accession No. P17921, Nov. 1990.

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