Stock material or miscellaneous articles – Composite – Of carbohydrate
Patent
1995-10-20
1998-04-07
Le, H. Thi
Stock material or miscellaneous articles
Composite
Of carbohydrate
21050029, 210656, 210730, 210777, 428533, 536 551, 536 553, 5361231, 536127, B32B 2304, C07H 504
Patent
active
057362599
DESCRIPTION:
BRIEF SUMMARY
FIELD OF INDUSTRIAL APPLICATION
The present invention relates to a packing material for high-performance liquid chromatography and a process for producing it. In particular, the present invention relates to a packing material for high-performance liquid chromatography, which comprises a polysaccharide derivative coated on a carrier and which is characterized in that the amount of a dissolved matter derived from the polysaccharide derivative is small, and a process for producing it.
The packing material for high-performance liquid chromatography of the present invention contributes to an improvement in the quality of a separated optical isomer in the field of separation of optical isomers. The present invention provides also a process for easily producing a packing material for high-performance liquid chromatography, which only produces a small amount of a dissolved matter, without reducing the separating capacity and at a low cost by coating a carrier with a polysaccharide derivative and removing dissolved matter derived from the polysaccharide derivative with a solvent.
PRIOR ART
Packing materials for high-performance liquid chromatography can be roughly classified into those of the chemical bond type and those of the coating type.
In the packing materials of the chemical bond type, the amount of dissolved matter from a substance having the separating capacity, is usually small, since this substance is chemically bonded to a carrier.
On the other hand, a solvent in which the substance having the separating capacity is soluble cannot be used for the packing materials of the coating type, since this substance is physically absorbed on a carrier and, in addition, there is a high possibility of the peeling and dissolution of this substance depending on the process conditions. The formation of the dissolved matter should be avoided, since it causes a drift phenomenon on the base line in a chromatogram during the analysis, and it causes contamination of the separated product.
The packing material for high-performance liquid chromatography, which comprises a polysaccharide derivative coated on a carrier, is suitable for the separation of an optical isomer, and it is known that the separation capacity thereof is very high (see Japanese Patent Laid-Open Nos. 082,858/1985, 108,751/1985, 142,930/1985, etc.). However, the packing material for high-performance liquid chromatography, which comprises a polysaccharide derivative coated on a carrier, has defects in that a polymer of a relatively low molecular weight part of the polysaccharide derivative is dissolved to make the base line of the chromatogram unstable, depending on the mobile phase used, that the dissolved matter contaminates the separated product during the course of the separation and that the separation capacity deteriorates during the course of use for a long period of time.
Under these circumstances, there is a demand for the development of a packing material for high-performance liquid chromatography, which is a polysaccharide derivative coated on a carrier which forms a greatly reduced amount of the dissolved matter.
DISCLOSURE OF THE INVENTION
After intensive investigations made for the purpose of solving these problems, the inventors have found that the intended packing material can be obtained without reducing the separating capacity by coating a carrier with a polysaccharide derivative and then washing the obtained product with a specified solvent to remove dissolved matter derived from the polysaccharide derivative. The present invention has been completed on the basis of this finding.
The present invention provides a packing material for high-performance liquid chromatography, which comprises a polysaccharide derivative coated on a carrier and which is characterized in that the amount of the dissolved matter derived from the polysaccharide derivative is small.
Preferably, the amount of the dissolved matter derived from the polysaccharide is not more than 0.1 mg as determined by passing a mixed solution of n-hexane/2-propanol in a volume
REFERENCES:
patent: 2978446 (1961-04-01), Battista et al.
patent: 3141875 (1964-07-01), Battista et al.
patent: 4912205 (1990-03-01), Okamoto et al.
patent: 5051176 (1991-09-01), Miyano et al.
patent: 5202433 (1993-04-01), Okamoto et al.
patent: 5354852 (1994-10-01), Ikeda
patent: 5489387 (1996-02-01), Namikoshi et al.
patent: 5491223 (1996-02-01), Okamoto
patent: 5496437 (1996-03-01), Okamoto et al.
Murata Kiyokazu
Oda Hiroshi
Daicel Chemical Industries Ltd.
Le H. Thi
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