Ornithine carbamoyltransferase

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

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4352523, 4353201, 435325, 536 232, C12N 910, C12N 120, C12N 1500, C07H 2104

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active

061657638

ABSTRACT:
The invention provides ornithine carbamoyltransferase polypeptides and DNA (RNA) encoding ornithine carbamoyltransferase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ornithine carbamoyltransferase polypeptides to screen for antibacterial compounds.

REFERENCES:
patent: 5434079 (1995-07-01), Mozayeni
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Kuo, L.C. et al. "Site-directed mutagenesis of Escherichia coli ornithine transcarbamoylase: role of arginine-57 in substrate binding and catalysis." Biochemistry (1988), vol. 27, pp. 8823-8832.
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Lane, D.P., GenBank Submission, Accession No. X52647, Direct Submission. (1990).
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MARSHALL, M., et al, "Ornithine transcarbamylase from Streptococcus faecalis and bovine liver. II. Multiple binding sites for carbamyl-P and L-norvaline, correlation with steady state kinetics" Journal of Biological Chemistry, Mar. 225, 1972, 247 (6) pp. 1654-1668.
MARSHALL, M., et al, "Ornithine transcarbamylase from Streptococcus faecalis and bovine liver. III. Multiple binding sites for carbamyl-P and L-norvaline, correlation with steady state kinetics" Journal of Biological Chemistry, Mar. 225, 1972, 247 (6) pp. 1669-1682.
TRICOT, C., et al. "Evolutionary relationships among bacterial carbamoyltransferases" Journal of General Microbiology, Sep. 1989, 135 (Pt 9) 2453-2464.
LANGE, R. et al. "Streptococcus pneumoniae rr 13 and hk 13 gene; two component system" GENEMBL Database Accession Number AJ006401.
EP Search Report from corresponding European Patent application no. 98203571.

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