Optical autofocus for use with microtiter plates

Details Optical autofocus for use with microtiter plates Optical autofocus for use with microtiter plates

1999-02-05

2000-10-10

Font, Frank G.

Optics: measuring and testing

Focal position of light source

356244, G01J 100

Patent

active

061307459

ABSTRACT:
A method and apparatus for autofocus on a target layer contained within a microplate well is provided. The instrument is capable of optically sensing a reference point on the underside of a microplate. This reference point is then used to focus light onto a target layer within the microplate well, the target layer having a location that is in defined relation to the reference point. The reference point is either a surface of the bottom of the microplate well or is an optically detectable mark on the underside of the microplate. In an alternate embodiment, a light position sensitive detector is used to enable deterministic autofocus for a plurality of wells on a microplate.

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Brochure, "Position Sensing Detectors Theory of Operation", Photonics, Inc., Lake Forest, California 92630.
Brochure, "Metallic Neutral Density Filters", Newport.

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