Oligonucleotides for detecting bacteria

Details Oligonucleotides for detecting bacteria Oligonucleotides for detecting bacteria

1992-08-19

1995-11-21

Parr, Margaret

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 237, 536 2432, C07H 2104

Patent

active

054688523

ABSTRACT:
Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

REFERENCES:
Nishibuchi et al., Infect. Immun 57(9): 2691-2697, 1989.
Nishibuchi et al. Mol Microbiol 4(1) 87-100, 1990.

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