Oligonucleotide signalling conjugate

Details Oligonucleotide signalling conjugate Oligonucleotide signalling conjugate

1994-09-22

1998-09-01

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

530402, 530404, 536 243, 536 2532, C12Q 168, C07H 2100, C07H 2102, C07H 2104

Patent

active

058009850

ABSTRACT:
Oligonucleotide signalling conjugates including a nucleic acid sequence, an amino-group containing linker group, a sulphur (thio) containing group and a non-isotopic label or marker and thiolated oligonucleotide derivative intermediates reactive with activated non-isotopic label or marker are described. The non-isotopic label is especially an enzyme such as alkaline phosphatase or horse radish peroxidase. The conjugates have application in the detection or characterisation of nucleic acid sequences and in particular in genetic characterisation.

REFERENCES:
patent: 5441867 (1995-08-01), Garman et al.
Guar et al, Nucl. Acids Res., V. 17, 1989, p. 4404.
Yoshitake et al, Eur. J. Biochem. V. 101 (1979) pp. 395-399.
Garman et al, Chemical Abstracts, 115:251622s.
Bischoff et al., "Introduction of 5'-Terminal Functional Groups into Synthetic Oligonucleotides for Selective Immobilization", Analytical Biochemistry, vol. 164, 1987, pp. 336-344.
Li et al., "Enzyme-linked synthetic oligonucleotide probes: non-radioactive detection of enterotoxigenic Escherichia coli in faecal specimens", Nucleic Acids Research, Vo. 15, No. 13, 1987, pp. 5275-5287.
Jablonski et al., "Preparation of Oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes", Nucleic Acids Research, vol. 14, No. 15, 1986, pp. 6115-6129.

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