Oligonucleotide probes complementary to and methods for distingu

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 29, 435 34, 436501, 935 77, 935 78, 536 26, 536 27, 536 28, C12Q 168, C12Q 102, C01N 33566, C07H 1906

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050343157

ABSTRACT:
Single-stranded oligonucleotide probes complementary to a signature region of Treponema hyodysenteriae (T. hyo) 16S rRNA are useful in distinguishing this pathogenic species of Treponema from closely related, nonpathogenic species. The probes find practical application in diagnosing swine dysentary from clinical samples, such as fecal material. They are also useful in differentiating T. hyo strains based on RFLP analysis.

REFERENCES:
Joens et al., The Diagnosis of Swine Dysentery Using a Labeled Nucleic Acid Probe., Proc. Intl. Ag. vol. Soc. 10th Cong., Rio de Janiero, Brazil, 1988.
Neil S. Jensen et al., "Detection of Treponema Hyodysenteriae, the Agent of Swine Dysentery. . ." Abstr. D-234, Abstracts of 89th Ann. Mtg. of American Society ofr Microbiology, New Orleans, LA, 5/14-18/89.
Ulf B. Gobel et al., "Oligonucleotide Probes Complementary to Variable Regions of Ribosomal RNA Discriminate Between Mycoplasma Species", J. Gen. Microbiol. 133: 1969-1974 (1987).
G. Haun et al., "Oligonucleotide Probes for Genus-, Species- and Subspecies-Specific Identification of Representatives of the Genus Proteus," FEMS Microbiol. Lett. 43: 187-193 (1987).

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