Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2008-01-29
2008-01-29
Lilling, Herbert J. (Department: 1657)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S037000, C435S039000, C435S042000, C435S232000, C435S252300, C435S252310, C435S252500, C435S253600, C435S254400, C424S093400
Reexamination Certificate
active
07323328
ABSTRACT:
The present invention relates to a novel obligately symbiotic thermophileSymbiobacterium toebiiSC-1 (Accession NO: KCTC 0685BP), and a thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced bySymbiobacterium toebiiSC-1. The present invention also relates to a pure culture method ofSymbiobacterium toebiiSC-1, a method of growth measurement forSymbiobacterium toebiiSC-1 and its relative symbiotic bacteria showing low-growth yield using nitrate respiration, and a screening method of relative symbiotic strains ofSymbiobacterium toebiiSC-1 from the environment using a specific antibody to it. Therefore, the thermostable L-tyrosine phenol-lyase and L-tryptophan indole-lyase produced bySymbiobacterium toebiiSC-1 in this invention can provide more stable catalysts in enzymatic biotransformation processes which enzymatically produce valuable medico-amino acids 3,4-dehydroxy-L-phenylalanine (L-DOPA) and L-tryptophan.
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Seung-Goo Lee et al., “Thermostable Tyrosine Phenol-Lyase of Symbiobacterium sp. SC-1: Gene Cloning, Sequence Determination, and Overproduction inEscherichia coli,” Protein Expression and Purification 11:263-270 (1997).
Michiyo Ohno et al., “Establishing the Independent Culture of a Strictly Symbiotic BacteriumSymbiobacterium thermophilumfrom Its Supporting Bacillus Strain,” Biosci. Biotechnol. Biochem., 63(6):1083-1090 (1999).
Bae Jin-Woo
Hong Seung-Pyo
Jeon Che Ok
Kang Dae-Joong
Kim Chul-Joong
Gates & Cooper LLP
Korea Research Institute of Bioscience and Biotechnology
Lilling Herbert J.
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