Nucleic acids encoding ara h 3 polypeptides

Details Nucleic acids encoding ara h 3 polypeptides Nucleic acids encoding ara h 3 polypeptides

2002-08-26

2009-02-03

Szperka, Michael (Department: 1644)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S325000, C530S370000, C530S806000, C530S868000

Reexamination Certificate

active

07485708

ABSTRACT:
It has been determined that allergens, which are characterized by both humoral (IgE) and cellular (T cell) binding sites, can be modified to be less allergenic by modifying the IgE binding sites. The IgE binding sites can be converted to non-IgE binding sites by masking the site with a compound that prevents IgE binding or by altering as little as a single amino acid within the protein, most typically a hydrophobic residue towards the center of the IgE binding epitope, to eliminate IgE binding. The method allows the protein to be altered as minimally as possible, other than within the IgE-binding sites, while retaining the ability of the protein to activate T cells, and, in some embodiments by not significantly altering or decreasing IgG binding capacity. The examples use peanut allergens to demonstrate alteration of IgE binding sites. The critical amino acids within each of the IgE binding epitopes of the peanut protein that are important to immunoglobulin binding have been determined. Substitution of even a single amino acid within each of the epitopes led to loss of IgE binding. Although the epitopes shared no common amino acid sequence motif, the hydrophobic residues located in the center of the epitope appeared to be most critical to IgE binding.

REFERENCES:
patent: 3645852 (1972-02-01), Axen et al.
patent: 4171299 (1979-10-01), Hamburger
patent: 4338297 (1982-07-01), Michael et al.
patent: 3720760 (1984-02-01), Bennich et al.
patent: 4469677 (1984-09-01), Michael et al.
patent: 4535010 (1985-08-01), Axen et al.
patent: 4579840 (1986-04-01), Hahn
patent: 4658022 (1987-04-01), Knowles et al.
patent: 4659678 (1987-04-01), Forrest et al.
patent: 4696915 (1987-09-01), Horecker
patent: 4816449 (1989-03-01), Hahn
patent: 4849337 (1989-07-01), Calenoff et al.
patent: 4849404 (1989-07-01), Iwai et al.
patent: 4900556 (1990-02-01), Wheatley et al.
patent: 4959314 (1990-09-01), Mark et al.
patent: 5026545 (1991-06-01), Saint-Remy et al.
patent: 5049390 (1991-09-01), Wojdani
patent: 5061790 (1991-10-01), Elting et al.
patent: 5091318 (1992-02-01), Anawis et al.
patent: 5169933 (1992-12-01), Anderson et al.
patent: 5314991 (1994-05-01), Oka et al.
patent: 5328991 (1994-07-01), Kuo
patent: 5389368 (1995-02-01), Gurtiss, III
patent: 5449669 (1995-09-01), Metcalfe et al.
patent: 5480972 (1996-01-01), Avjioglu et al.
patent: 5486452 (1996-01-01), Gordon et al.
patent: 5496554 (1996-03-01), Oka et al.
patent: 5543144 (1996-08-01), Chang
patent: 5547669 (1996-08-01), Rogers et al.
patent: 5558869 (1996-09-01), Burks, Jr. et al.
patent: 5583046 (1996-12-01), Valenta et al.
patent: 5591433 (1997-01-01), Michael et al.
patent: 5597895 (1997-01-01), Gaynor et al.
patent: 5616559 (1997-04-01), Androphy et al.
patent: 5625039 (1997-04-01), Washida et al.
patent: 5637454 (1997-06-01), Harley
patent: 5648242 (1997-07-01), Valenta et al.
patent: 5652122 (1997-07-01), Frankel et al.
patent: 5667965 (1997-09-01), Androphy et al.
patent: 5670617 (1997-09-01), Frankel et al.
patent: 5674980 (1997-10-01), Frankel et al.
patent: 5693495 (1997-12-01), Breiteneder et al.
patent: 5710126 (1998-01-01), Griffith et al.
patent: 5731157 (1998-03-01), Miller et al.
patent: 5736149 (1998-04-01), Avjioglu et al.
patent: 5747641 (1998-05-01), Frankel et al.
patent: 5759572 (1998-06-01), Sugimoto et al.
patent: 5773003 (1998-06-01), Swain et al.
patent: 5786466 (1998-07-01), Breitenbach et al.
patent: 5804604 (1998-09-01), Frankel et al.
patent: 5807746 (1998-09-01), Lin et al.
patent: 5820862 (1998-10-01), Garman et al.
patent: 5820880 (1998-10-01), Alving et al.
patent: 5830463 (1998-11-01), Duke et al.
patent: 5837550 (1998-11-01), Breitenbach et al.
patent: 5843672 (1998-12-01), Morgenstern et al.
patent: 5843710 (1998-12-01), Cobon et al.
patent: 5869040 (1999-02-01), Oin
patent: 5888762 (1999-03-01), Joliot et al.
patent: 5888799 (1999-03-01), Curtiss, III
patent: 5891432 (1999-04-01), Hoo
patent: 5891716 (1999-04-01), Morgenstern et al.
patent: 5939283 (1999-08-01), Morgenstern et al.
patent: 5973121 (1999-10-01), Burks, Jr. et al.
patent: 5989814 (1999-11-01), Frankel et al.
patent: 5998583 (1999-12-01), Korsmeyer
patent: 6008340 (1999-12-01), Ball et al.
patent: 6060082 (2000-05-01), Chen et al.
patent: 6187311 (2001-02-01), Nishiyama et al.
patent: 6218371 (2001-04-01), Krieg et al.
patent: 6486311 (2002-11-01), Burks, Jr. et al.
patent: 2002/0187158 (2002-12-01), Mahler et al.
patent: 2 157 596 (1994-09-01), None
patent: 2 158 047 (1994-09-01), None
patent: 0 819 763 (1998-01-01), None
patent: 0684812 (1998-01-01), None
patent: 0877033 (1998-11-01), None
patent: 072 85875 (1994-04-01), None
patent: 06253851 (1994-09-01), None
patent: 07095887 (1995-04-01), None
patent: WO 90/04025 (1990-04-01), None
patent: WO 91/06571 (1991-05-01), None
patent: WO 91/11718 (1991-08-01), None
patent: WO 92/02621 (1992-02-01), None
patent: WO 92/03551 (1992-03-01), None
patent: WO 92/11859 (1992-07-01), None
patent: WO 93/21223 (1993-10-01), None
patent: WO 94/05303 (1994-03-01), None
patent: WO 94/10194 (1994-05-01), None
patent: WO 94/20614 (1994-09-01), None
patent: WO 94/23035 (1994-10-01), None
patent: WO 94/24281 (1994-10-01), None
patent: WO 95/07933 (1995-03-01), None
patent: WO 95/34578 (1995-12-01), None
patent: WO 96/36880 (1996-11-01), None
patent: WO 97/05265 (1997-02-01), None
patent: WO 97/24139 (1997-07-01), None
patent: WO 98/32866 (1998-07-01), None
patent: WO 98/43657 (1998-08-01), None
patent: WO 98/39029 (1998-09-01), None
patent: WO 99/16467 (1999-04-01), None
patent: WO 99/25387 (1999-05-01), None
patent: WO 99/38978 (1999-05-01), None
patent: WO 99/34826 (1999-07-01), None
patent: WO 99/38978 (1999-08-01), None
patent: WO 99/49879 (1999-10-01), None
patent: WO 00/54803 (2000-09-01), None
patent: WO 01/36621 (2001-05-01), None
Colman et al., Research in Immunology, vol. 145, No. 1, 1994, pp. 33-36.
Till et al., in Allergens and Allergen Immunotherapy, third edition, Marcel Dekker, Inc., 2004, pp. 85-104.
Rabjohn et al., “Glycinin, a third major peanut allergen identified in soy-adsorbed serum IgE from peanut sensitive individuals” J. Allergy Clin. Immunol., Jan. 1998, vol. 101, No. 1 Part 2, pp. S240.
Blumenthal et al., in Allergens and Allergen Immunotherapy, 3rd edition, 2004, Marcel Dekker, pp. 37-50.
Burks et al., Eur J Biochem, 1997, 245:334-339.
Reese et al., J Immunol, 2005, 175:8354-8364.
Andrews, et al.,Gene, 182(1-2): 101-9, 1996.
Bolhaar et al., Clin Exp Allergy, 35(12): 1638-44, 2005.
Dizier et al.,Genetic Epidemiology, 16: 305-15, 1999.
Ferreira et al.,J. Exp. Med., 183: 599-609, 1996.
Ferreira et al.,The FASEB Journal, 12: 231-42, 1998.
Gadermaier et al.,Int Arch Allergy Immunol., 139(1): 53-62, 2006.
Harvey,Remington's Pharmaceutical Sciences, 18thed., Gennaro(ed), Mack Publishing Co., Easton, PA, 35: 697-724, 1990.
Holm, et al.,J. of Immunology, 173: 5258-5267, 2004.
Kraft et al.,Int Arch Allergy Immunol, 118: 171-76, 1999.
Li, et al.,The Journal of Immunology, 162: 3045-3052, 1999.
Schramm et al.,The J. of Immunology, 162: 2406-2414, 1999.
Scheurer et al.,Eur J Nutr., 38(4): 201-15, 1999.
Swoboda et al.,Eur J Immunol, 32(1): 270-80, 2002.
Walker,Vaccine, 12(5): 387-400, 1994.
Chatel, et al., “Various Factors (Allergen Nature, Mouse Strain, CpG/Recombinant Protein Expressed) Influence the Immune Response Elicited by Genetic Immunization”,Allergy, 58: 641-647, 2003.
Evans, et al., “Non-Replicating Oral Whole Cell Vaccine Protective Against EnterotoxigenicEscherichia coli(ETEC) Diarrhea: Stimuation of Anti-CFA (CFA/I) and Anti-Enterotoxin (Anti-LT) Intestinal IgA and Protection Against Challenge with ETEC Belonging to Heterologous Serotypes”,FEMS Microbiology Immunology, 47: 117-126, 1988.
Gotlieb, “Scientists Develop Vaccine Strategy for Peanut Allergy”,BMJ, 318: 894, 1999.
Vrtala, et al., “Conversion of the Major Birch Pollen Allergen, Bet v 1, into Two Nonanaphylactic T Cell Epitope-Containing Fragments”,J. Clin. Invest. 99(7): 1673-1681, 1997.
Del Val, et al., “Thioredoxin Treatment Increases Digestibility

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