Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2009-07-30
2011-12-06
Swope, Sheridan (Department: 1652)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S320100, C435S252300, C435S070100, C435S071100
Reexamination Certificate
active
08071736
ABSTRACT:
Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
REFERENCES:
patent: 6060594 (2000-05-01), English et al.
patent: 7473821 (2009-01-01), Abad
patent: 2003/0120054 (2003-06-01), Chen et al.
patent: 2004/0096934 (2004-05-01), Freyssinet et al.
patent: 0 358 557 (1990-03-01), None
patent: WO 98/21348 (1998-05-01), None
patent: WO 02/34774 (2002-05-01), None
patent: WO 03/018810 (2003-03-01), None
patent: WO 2004/003148 (2004-01-01), None
USPTO in house alignment Seq Id No. 4 vs Q70EX2 from Bown et al, Insect Biochem Mol Biol. Apr. 2004;34(4):305-20 (IDS). Performed May 30, 2011.
Angsuthanasombat, et al.,J. Biochem. Mol. Biol., 2001, pp. 402-407, vol. 34.
Clarke, et al., Physiol. Plant., 2005, pp. 359-361, vol. 123.
De Maagd, et al.,Appl. Environ. Microbiol., 1999, pp. 4369-4374, vol. 65.
Guo, et al.,Proc. Natl. Acad. Sci., USA, 2004, pp. 9205-9210, vol. 101.
Hill, et al.,Biochem. Biophys. Res. Comm., pp. 573-577, vol. 244.
Koiwa, H., et al. “A plant defensive cystain (soycystain) targets cathesin L-like digestive cysteine proteinase (DvCALs) in the larval midgut of western corn rootworm (Diabrotica virgifera virgifera),”FEBS Letters, 2000, pp. 67-70, vol. 471.
Lazar, et al.,Mol. Cell. Biol., 1988, pp. 1247-1252, vol. 8.
Miranda, R., et al. “Processing of Cry 1 Abδ-endotoxin fromBacillus thuringiensisbyManduca sextaandSpodoptera frugiperdamidgut proteases: role in protoxin activation and toxin inactivation”,Insect Biochemistry and Molecular Biology, 2001, pp. 1155-1163, vol. 31.
Moellenbeck, D, J., et al. “Insecticidal proteins fromBacillus thuringiensisprotect corn from corn rootworms,”Nature Biotechnology, Jul. 2001, pp. 668-672, vol. 19.
Outchkourov, N, S., et al. “Expression of Sea Anemone Equistatin in Potato. Effects of Plant Proteases on Heterologous Protein Production,”Plant Physiology, September.
Rukmini, V., et al. “Bacillus thuringiensiscrystal δ-endotoxin: Role of proteases in the conversion of protoxin to toxin,”Biochimie, 2000, pp. 109-116, vol. 82.
Tounsi, et al.,J. Appl. Microbiol., pp. 23-28, vol. 95.
Bown, D.P., et al. “Characterisation of Cysteine Proteinases Responsible for Digestive Proteolysis in Guts of Larval Western Corn Rootworm (Diabrotica virgifera) by Expression in the YeastPichia pastoris,” Insect Biochem. Mol. Biol., 2004, pp. 305-320, vol. 4.
NCBI Report for Database Accession No. AJ583508.1, From Bown et al, Insect Biochem Mol Biol 2004 34:305-320.
NCBI Report for Database Accession No. AJ583508.1, Alignment with SEQ ID No. 2, From Bown et al. Insect Biochem Mol Biol 2004 34:305-320.
Genbank Report for Accession No. CAD57542, Direct Submission, Nov. 23, 2002.
Crickmore, Neil et al. Web <2010, http://www.lifesci.sussex.ac.uk/home
eil—Crickmore/Bt/>.
Abad Andre R.
Flannagan Ronald D.
Herrmann Rafael
Lu Albert L.
McCutchen Billy F.
Alston & Bird LLP
E.I. Dupont de Nemours and Company
Pioneer Hi-Bred International , Inc.
Swope Sheridan
LandOfFree
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