Nucleic acid adapters containing a type IIs restriction site and

Details Nucleic acid adapters containing a type IIs restriction site and Nucleic acid adapters containing a type IIs restriction site and

1998-01-16

2000-02-22

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9141, 536 241, 536 242, 536 2433, C12Q 168

Patent

active

060278943

ABSTRACT:
The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the "capturing" and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.

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