Nuclease

Details Nuclease Nuclease

2005-05-17

2005-05-17

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C536S023200

Reexamination Certificate

active

06893854

ABSTRACT:
A process for producing a polynucleotide encoding a restriction endonuclease with an altered specificity, which process comprises:(a) mutagenising a polynucleotide encoding a restriction endonuclease with specificity for a recognition sequence so as to produce one or more mutated polynucleotides; and(b) isolating therefrom a polynucleotide encoding a mutated restriction endonuclease with specificity for an altered recognition sequence by selecting a polynucleotide which expresses a restriction endonuclease with methylase specificity for the altered recognition sequence.

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