Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1985-03-01
1988-07-12
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4351723, 435320, 935 44, 935 45, C12N 120, C12N 1500, C12N 100
Patent
active
047570202
ABSTRACT:
Expression plasmids containing the full cDNA sequence of calf prochymosin and capable of expressing prochymosin gene in E. coli host cells are disclosed. A method is described for the preparation of said plasmids which comprises altering the spacing between the SD and ATG sequences within the E. coli trp promoter-operator region of the parent expression plasmid, pCR 701, which is known to express prochymosin gene in the E. coli host cells. These modified expression plasmids are designed to provide high expression levels of prochymosin.
REFERENCES:
Nishimori et al, Gene, vol. 19, pp. 337-344, 1982.
Goff et al, Gene, vol. 27, pp. 35-46, 1984.
Fujisawa et al, Nuclei Acids Research, vol. 11, pp. 3581-3591, Jun. 1983.
Tacon et al, Molec. Gen. Genet., vol. 177, pp. 427-438, (1980).
Guarente et al, Cell, vol. 20, pp. 543-553, Jun. 1980.
Roberts et al, PNAS U.S.A., vol. 76, pp. 760-764, Feb. 1979.
Maniatis et al, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Lab., pp. 411-417, (1982).
Abstract of the Japanese Agricultural Chemistry Society Meetings, 2A-19 (1983).
Beppu Teruhiko
Kawaguchi Yoshiyuki
Nishimori Katsuhiko
Shimizu Norio
Uozumi Takeshi
Beppu Teruhiko
Tanenholtz Alvin E.
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