Chemistry: analytical and immunological testing – Biological cellular material tested
Reexamination Certificate
2011-03-01
2011-03-01
Griffin, Walter D (Department: 1797)
Chemistry: analytical and immunological testing
Biological cellular material tested
C514S354000, C514S277000, C514S183000
Reexamination Certificate
active
07897400
ABSTRACT:
This invention relates to a test for detecting aMycobacterium tuberculosis(tuberculosis or TB) infection in a patient or subject, specifically a diagnostic test, including a breath test, whereby patients are provided a small dose of an isotopically labeled TB drug, Isoniazid (INH) orally or directly to the lungs of the patient or subject. If TB is present, a TB enzyme mycobacterial peroxidase KatG oxidizes the INH; and KatG specific metabolites, in particular, isotopically labeled nitric oxide (NO), nitrites, nitrates, carbon monoxide (CO) or carbon dioxide converted from carbon monoxide of INH cleavage are measured. Other embodiments relate to a diagnostic breath test for detecting TB utilizing isotopically labeled urea (preferably, carbon-13 labeled urea), alone or in combination with isotopically labeled isoniazid (preferably, nitrogen-15 labeled isoniazid), whereinM. tuberculosisorganism, if present in the patient or subject's lungs (or other tissues), will metabolize the isotopically labeled urea to isotopically labeled carbon dioxide (CO2) such that a determination of the residence ofM. tuberculosis, including residence of an isoniazid resistant strain ofM. tuberculosis, may be made.
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Deretic Vojo P.
Timmins Graham
Coleman Henry D.
Griffin Walter D
Mui Christine T
Sapone William J.
STC.UNM
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