Nicotinamide-adenine-dinucleotide chemically coupled to water-in

Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal

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195 63, 195 68, 1951035R, 260141, 260142, 260156, C07C10700, C07G 702

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active

039577481

ABSTRACT:
Nicotinamide-adenine-dinucleotide (NAD) can be coupled chemically to an essentially water-insoluble carrier material selected from the group consisting of glass particles having a diazotizable silane coupling agent attached thereto, p-aminobenzylcellulose, polyaminopolystyrene, and the half amide of benzidine-carboxymethylcellulose by diazotizing the carrier materials and reacting the diazotized materials with a solution of NAD. Methods of chemically coupling the NAD to such carriers are disclosed and the redox activity of NAD coupled to porous glass beads through an intermediate silane coupling agent is demonstrated.

REFERENCES:
patent: 3519538 (1970-07-01), Messing et al.
patent: 3556945 (1971-01-01), Messing
patent: 3574062 (1971-04-01), Sato
Sexton, "Chemical Constitution and Biological Activity", pp. 23-27 (1949).
Webb, "Enzymes", pp. 394-398 (1958).
Kay, Chemical Abstracts, Vol. 69, p. 7049 (1968).
Wilkinson, "Introduction to Diagnostic Enzymology", pp. 9-12 (1962).

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