NAD(P)-Independent glycerol dehydrogenase, a process for the pre

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435190, 435822, 435823, C12Q 100, C12Q 132, C12N 904, C12R 101, C12R 102

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045769133

ABSTRACT:
The present invention provides a new NAD(P)-independent glycerol dehydrogenase and a process for obtaining it, wherein a micro-organism of the species Pseudomonas, Serratia, Klebsiella, Erwinia, Aspergillus, Penicillium, Rhizopus, Acetobacter or Gluconobacter which is capable of forming this enzyme is cultured in a nutrient medium and the enzyme isolated from the culture broth.
The present invention also provides processes for the determination of glycerol and of triglycerides which make use of this new enzyme.

REFERENCES:
patent: 2948658 (1960-08-01), Green
patent: 4223090 (1980-09-01), Mazza
Chemical Abstracts: 91: 106580h Yamada, et al., J. Ferment. Technol. 1979, 57(3) 221-6.
Hemme, D. et al., Chemical Abstracts I: 94(19): 152446d (1981).
Sarkar, S. R., Chemical Abstracts II: 98(13) :105259t (1982).
Yamada, Shigeki et al., Chemical Abstracts III: 88(1): 2162v (1977).
Merck Index, ninth edition, pp. 2511, 6170 and 5192.

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