Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1987-11-17
1990-10-02
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435850, C12N 904
Patent
active
049607014
ABSTRACT:
N-Acetylmannosamine dehydrogenase which takes off hydrogen from N-acetylmannosamine to convert it into N-acetylmannosaminolactone and, at the same time, reduces coenzyme NAD into NADH. This enzyme can be obtained by culturing, in a medium, a strain belonging to Genus Flavobacterium and having an ability to produce N-acetylmannosamine dehydrogenase producing activity, and then collecting it. This enzyme is usable in the quantitative analysis of N-acetylmannosamine or sialic acid.
REFERENCES:
Yoneyama et al., "Distribution of Mannosamine and Mannosaminuronic Acid Among Cell Walls of Bacillus Species," J. Bacterial., vol. 149, No. 1, pp. 15-21, 1982.
De-Buch: H. U. Bergmeyer, Methods of Enzymatic Analysis, vol. VI, Verlag Chemie, Weinheim 1984, S. 80 bis 90.
Patent Abstracts of Japan C-351, Jun. 5, 1986, vol. 10/No. 15: 61-12299 (A).
Patents Abstracts of Japan C-327, Feb. 6, 1986, vol. 10/No. 3: 60-184 399 (A).
Methods in Enzymology (1963), vol. VI, pp. 465-467.
Clinica Chimica Acta, International Journal of Clinical Chemistry, vol. 108/1 (1980).
Horiuchi Tatsuo
Kurokawa Toshiko
Noda Institute for Scientific Research
Wax Robert A.
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