N-acetylgalactosaminyl or N-acetylglucosaminyl transfer using N-

Details N-acetylgalactosaminyl or N-acetylglucosaminyl transfer using N- N-acetylgalactosaminyl or N-acetylglucosaminyl transfer using N-

1993-09-13

1996-05-14

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 72, 435 89, 435 94, 435100, 435101, C12P 1918, C12P 1900, C12P 1930, C12P 1924

Patent

active

055166652

ABSTRACT:
A one-pot glycosylation reaction is disclosed in which a N-acetylgalactosamine (GalNAc) or N-acetylglucosamine (GlcNAc) group is enzymatically transferred to an acceptor molecule. The starting glycoside is a N-acetylamino monosaccharide 1-phosphate that is enzymatically converted to its UDP derivative via UTP and a pyrophorylase. The formed UDP derivative is epimerized, and the epimerized UDP derivative is used in the enzyme-catalyzed glycosyl transfer. That enzyme-catalyzed glycosyl transfer to an acceptor releases UDP that is enzymatically converted to UTP for further conversion of the N-acetylamino monosaccharide 1-phosphate into its UDP derivative.

REFERENCES:
patent: 4569909 (1986-02-01), Seno et al.
patent: 5068191 (1991-11-01), Clausen et al.
patent: 5180674 (1993-01-01), Roth
patent: 5246840 (1993-09-01), Nilsson
patent: 5264352 (1993-11-01), Thiem et al.
Pure & Appl. Chem. 65(4): pp. 803-808 (1993), Wong et al.
J. Am. Chem. Soc. 113(16): pp. 6300-6302, (1991), Ichikawa et al.
J. Org. Chem. 47(27): pp. 5416-5418 (1982), Wong et al.

Affiliated with

Also associated with

Rating

N-acetylgalactosaminyl or N-acetylglucosaminyl transfer using N- does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with N-acetylgalactosaminyl or N-acetylglucosaminyl transfer using N-, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and N-acetylgalactosaminyl or N-acetylglucosaminyl transfer using N- will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1895070