Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Digestive system
Patent
1999-05-04
2000-08-01
Witz, Jean C.
Drug, bio-affecting and body treating compositions
Extract, body fluid, or cellular material of undetermined...
Digestive system
623 2, 623 11, 623 14, A61K 3538
Patent
active
060963476
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to a tissue graft composition and methods for its preparation and use. More particularly, the present invention is directed to non-immunogenic submucosal tissue graft compositions prepared from warm-blooded vertebrates and the use of those compositions to promote growth of endogenous cardiac tissues.
BACKGROUND AND SUMMARY OF THE INVENTION
It is known that compositions comprising the tunica submucosa of the intestine of warm-blooded vertebrates can be used as tissue graft materials. See U.S. Pat. Nos. 4,902,508 and 5,281,422. The tissue graft compositions described in those patents are characterized by excellent mechanical properties, including a high burst pressure, and an effective porosity index which allows such compositions to be used beneficially for vascular graft and connective tissue graft constructs. When used in such applications the graft constructs appear not only to serve as a matrix for the regrowth of the tissues replaced by the graft constructs, but also promote or induce such regrowth of endogenous tissue. Common events to this remodeling process include: widespread and very rapid neovascularization, proliferation of granulation mesenchymal cells, biodegradation/resorption of implanted intestinal submucosal tissue material, and absence of immune rejection.
It is also known that intestinal submucosa can be fluidized by comminuting and/or enzymatic digestion, without loss of its apparent biotropic properties, for use in less invasive methods of administration (e.g., by injection or topical application) to host tissues in need of repair. See U.S. Pat. No. 5,275,826.
Surprisingly, it has been found that submucosal tissue is also capable of promoting endogenous regrowth and healing of damaged or diseased cardiac tissues, including the endocardium, pericardium, and myocardium. Myocardial tissue comprises the middle muscular tissue of the heart wall. Cardiac muscle tissue differs from smooth and skeletal muscle in that the nuclei are centrally located, the cells form a syncytium, and the cells exhibit the property of spontaneous contractibility or "automaticity."
In accordance with the present invention submucosal tissue of a warm blooded vertebrate is used for the preparation of a graft construct that promotes endogenous regrowth and healing of damaged or diseased cardiac tissues upon contact of said damaged or diseased tissues with the composition.
The present submucosal tissue graft compositions can be implanted or injected into a vertebrate host to induce the repair or replacement of damaged or defective heart tissues. In one embodiment damaged or diseased myocardial tissues are contacted in vivo with a composition comprising intestinal tunica submucosa of a warm blooded vertebrate to enhance the formation of endogenous tissues having spontaneous contractile properties.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 illustrates myographic data for remodeled canine ventricular myocardium. Spontaneous contractions (1, 2 and 3) and an evoked contraction (50 ms, 160 mA) were observed in the remodeled tissue from the MV2 dog.
FIG. 2 is a graphic representation of strength-duration data for remodeled canine ventricular myocardium of the MV2 dog (Rheobase=55.42 mA).
FIG. 3 illustrates myographic data for remodeled canine atrial septum in the MA1 dog when the tissue was exposed to electrical stimulation (50 ms, 160 mA).
FIG. 4 is a graphic representation of strength-duration data for remodeled canine atrial septal tissues of the MA2 dog (Rheobase=30.9 mA).
FIG. 5 is a graphic representation of strength-duration data for remodeled canine ventricular myocardium of the MV1 dog (Rheobase=139 mA).
FIG. 6 is graphic representation of strength-duration data for normal canine ventricular tissue of the MV1 dog (Rheobase=55.42 mA).
DETAILED DESCRIPTION OF THE INVENTION
There is provided in accordance with this invention a method and composition for promoting the repair of damaged or diseased heart tissues. Generally the method comprises the step of cont
REFERENCES:
patent: 5480424 (1996-01-01), Cox
"Fascia lata replacement of aortic valves," The Journal of Thoracic And Cardiovascular Surgery, Ake Senning, vol. 54, No. 4, Oct. 1967, pp. 465-470.
"Mitral and aortic valve replacement with fascia lata on a frame," The Journal of Thoracic And Cardiovascular Surgery, W. Sterling Edwards, et al., vol. 58, No. 6, Dec. 1969, pp. 854-858.
Badylak Stephen F.
Geddes Leslie A.
Havel William J.
Matheny Robert G.
Obermiller Fred J.
Purdue Research Foundation
Witz Jean C.
LandOfFree
Myocardial graft constructs does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Myocardial graft constructs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Myocardial graft constructs will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-661219