Mycobacteriophage DSGA specific for the mycobacterium tuberculos

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 221, 536 231, 536 2432, C12Q 168, C12P 1934, C07H 2102, C07H 2104

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active

054767687

ABSTRACT:
Mycobacteriophage DS6A has been characterized and found to specifically infect all species of the TB complex, without any detectable infection of mycobacteria species other than those of the TB complex. DNA sequence analysis revealed several potential open reading frames, including one encoding a protein analogous to gp37 of mycobacteriophage L5 and a second encoding a protein with significant homology to the S. coelicolor DNA polymerase .beta. subunit. Based on the DNA sequence analysis, cloning sites can be identified for insertion of reporter genes, making DS6A useful as a reporter phage for specific detection and identification of species of the TB complex.

REFERENCES:
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Ross et al., (1991), "Differentiation of Mycobacterium tuberculosis strains by use of a nonradioactive southern blot hybridization method", J. Inf. Dis. 163:904-907.
Jones, (1988), "Bacteriophage typing of Mycobacterium tuberculosis cultures from incidents of suspected laboratory cross contamination" Tubercle 69:43-46.
Saunders, (1995), "State of the art: typing Mycobacterium tuberculosis", J. Hosp. Inf. 29::169-176.
Sula et al., (1979), "Accidental contamination of diagnostic cultures of mycobacteria and phage identification of the contaminating strain", Tubercle 60:159-162.
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Grange et al., (1978), "Host-phage relationships in the genus mycobacterium and their clinical significance", Tubercle 59:203-225.

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