Mutant F. turlarensis strain and uses thereof

Details Mutant F. turlarensis strain and uses thereof Mutant F. turlarensis strain and uses thereof

2011-03-22

2011-03-22

Graser, Jennifer E (Department: 1645)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S173300, C424S234100, C536S023700

Reexamination Certificate

active

07910351

ABSTRACT:
A mutant strain ofFrancisella tularensishas attenuated virulence and has a mutation in the gene coding for a putative peroxynitrite resistance protein A (prpA) which prevents normal function of the protein. The mutant is useful as a vaccine against type A and B virulent strains ofF. tularensis, and is produced by obtaining a virulentF. tularensisstrain and mutating the gene, FTT0918, that codes for prpA.

REFERENCES:
patent: WO 2004/003009 (2004-01-01), None
Twine, S. et al. A mutant ofFrancisella tularensisstrain SCHU S4 lacking the ability to express a 58-kilodalton protein is attenuated for virulence and is an effective live vaccine. Infection and immunity, Dec. 2005, vol. 73, No. 12, pp. 8345-8352.
Broekhuijsen, M. et al. Genome-wide DNA microarray analysis ofFrancisella tularensisstrains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulentF. tularensissubsp.tularensis. Journal of Clinical Microbiology, Jul. 2003, vol. 41, No. 7, pp. 2924-2931.
Svensson, K. et al. Evolution of subspecies ofFrancisella tularensis. Journal of Bacteriology, Jun. 2005, vol. 187, No. 11, pp. 3903-3908.
Larsson, P. et al. The complete genome sequence ofFrancisella tularensis, the causative agent of tularemia. Nature Genetics, Feb. 2005, vol. 37, No. 2, pp. 153-159.
Sjostedt, A. Virulence determinants and protective antigens ofFrancisella tularensis. Curr Opin Microbiol 2003; 6:66-71.
Eigelsbach, H.T. et al. Prophylactic effectiveness of live and killed tularemia vaccines I. Production of vaccine and evaluation in the white mouse and guinea pig. J Immunol 1961; 87:415-25.
Saslaw, S. et al. Tularemia vaccine study II. Respiratory challenge. Arch Int Med 1961; 107:702-14.
Saslaw, S. et al. Tularemia vaccine study I. Intracutaneous challenge. Arch Int Med 1961; 107:689-701.
Burke, D.S. Immunization against tularemia: Analysis of the effectiveness of liveFrancisella tularensisvaccine in prevention of laboratory-acquired tularemia. J Infect Dis 1977; 135(1):55-60.
Conlan, J.W. 2004. Vaccines againstFrancisella tularensis—past, present and future. Expert Review of Vaccines. 3:307-314.
Eigelsbach, H.T. et al. Aerogenic immunization of the monkey and guinea pig with live tularemia vaccine. Proc. Soc Exptl Biol Med 1961; 108:732-34.
Hornick, R.B. et al. Aerogenic immunization of man with live tularemia vaccine. Bact Revs 1966; 30(3):532-38.
Conlan, J.W. et al. Aerosol-, but not intradermal-immunization with the live vaccine strain ofFrancisella tularensisprotects mice against subsequent aerosol challenge with A highly virulent type A strain of the pathogen by an αβ T cell- and interferon gamma-dependent mechanism. Vaccine 2005; 23:2477-2485.
Johansson, A. et al. Evaluation of PCR-based methods for discrimination ofFrancisellaspecies and subspecies and development of a specific PCR that distinguishes the two major subspecies ofFrancisella tularensis. J Clin Micro 2000; 38(11):4180-85.
Golovliov, I. et al. A method for allelic replacement inFrancisella tularensis. FEMS Microbiol Lett 2003; 222 (2):273-280.
Conlan, J.W. et al. Experimental tularemia in mice challenged by aerosol or intradermally with virulent strains ofFrancisella tularensis: Bacteriologic and histopathologic studies. Microb Pathog 2003; 34:239-48.
Golovliov, I. et al. Identification of proteins ofFrancisella tularensisinduced during growth in macrophages and cloning of the gene encoding a prominently induced 23-kilodalton protein. Infect Immun 1997; 65(6):2183-2189.
Lai, X. et al. Expression of IgIC is necessary for intracellular growth and induction of apoptosis in murine macrophages byFrancisella tularensis. Microbial Pathogenesis 2004; 37:225-30.
Lindgren, H. et al. Factors affecting the escape ofFrancisella tularensisfrom the phagolysosome. J Med Micro 2004; 53:953-958.
Lauriano, C.M. et al. MgIA regulates transcription of virulence factors necessary forFrancisella tularensisintraamoebae and intramacrophage survival. PNAS 2004; 101(12): 4246-4249.
Database UniProt [Online], “SubName:Full=Putative uncharacterized protein”, Feb. 1, 2005, XP002526472 retrieved from EBI accession No. UNIPROT:Q5NGC7, Database accession No. G5NGC7.
Database Geneseq [Online], “Francisella tularensisimmunogenic protein 61, SEQ ID No. 61”, Mar. 25, 2004, XP002526473 retrieved from EBI accession No. GSP:ADH12880, Database accession No. ADH12880.
Rohmer, Laurence et al., “Potential source ofFrancisella tularensislive vaccine strain attenuation determined by genome comparison”, Infection and Immunity, vol. 74, No. 12, Dec. 2006, pp. 6895-6906, XP002526471, ISSN: 0019-9567.
EP 06 72 1840 Supplementary Search Report, Jun. 8, 2009.

Affiliated with

Also associated with

Rating

Mutant F. turlarensis strain and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Mutant F. turlarensis strain and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mutant F. turlarensis strain and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2621136