Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2005-03-11
2008-09-30
Zara, Jane (Department: 1635)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S069100, C435S194000, C435S199000, C536S023100, C536S023200
Reexamination Certificate
active
07429468
ABSTRACT:
The present invention relates to thermostable mutants of B-type DNA polymerases comprising a Y-GG/A amino acid motif between the N-terminal 3′-5′-exonuclease domain and the C-terminal polymerase domain whereas the tyrosine of the Y-GG/A amino acid motif is mutated and whereas these mutant DNA polymerases are suitable for PCR.
REFERENCES:
patent: 6881559 (2005-04-01), Sobek et al.
patent: 196 11 759 (1996-03-01), None
Anderson, WF., “Human Gene Therapy,”Nature, Apr. 1998, pp. 25-28, vol. 392.
Antranikian, G., et al., Accession DE19611759-A1, Apr. 1998.
Bohlke, K., et al., “PCR performance of the B-type DNA polymerase from the thermophilic euryachaeonThermococcus aggregansimproved by mutations in the Y-GG/A motif,”Nucleic Acids Research, 2000, pp. 3910-3917, vol. 28, No. 20.
Bordo, D., et al., “Suggestions for “Safe” Residue Substitutions in Site-directed Mutagenesis,”J. Mol. Biol., 1991, pp. 721-729, vol. 217.
Niehaus, F., et al., “Cloning and characterization of a thermostable x-DNA polymerase from the hyperthermophilic archaeonThermococcus sp. TY,”Gene, 1997, pp. 153-158, vol. 204.
Pisani, F., et al., “Amino Acid Residues involved in Determining the Processivity of the 3′-5′ Exonuclease Activity in a Family B DNA Polymerase from the Thermoacidophilic ArchaeonSulfolobus solfataricus,”Biochemistry, 1998, pp. 15005-15012, vol. 37.
Truniger, V., et al., “Role of the ‘YxGG/A’ Motif of 029 DNA Polymerase in Protein-primed Replication,”J. Mol. Biol. 1992, pp. 57-69, vol. 286.
Truniger, V., et al., “A DNA binding motif coordinating synthesis and degradation in proofreading DNA polymerases,”The Embo Journal, pp. 3430-3441, vol. 15, No. 13, (1996).
Antranikian Garabed
Frey Bruno
Kristina Boehlke
Pisani Francesca Maria
Rossi Mose
Roche Diagnostics GmbH
Townsend and Townsend / and Crew LLP
Zara Jane
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