Mutant AOX2 promoter, microorganism carrying same, method of pre

Details Mutant AOX2 promoter, microorganism carrying same, method of pre Mutant AOX2 promoter, microorganism carrying same, method of pre

1994-08-10

1997-03-11

Stanton, Brian R.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 691, 4351723, 43525423, 4353201, 536 241, 536 234, 935 28, 935 69, C07H 2104, C12N 116, C12N 119, C12P 2102

Patent

active

056100361

ABSTRACT:
A mutant AOX2 (alcohol oxidase 2) promoter derived from the natural AOX2 promoter by base sequence deletion, insertion, or substitution is disclosed. A microbial strain carrying a gene under the control of such a mutant AOX2 promoter can be obtained by growing in a methanol-containing medium a strain incapable of producing AOX encoded by the AOX1 gene but carrying the AOX2 gene under the control of the natural AOX2 promoter. A heterologous protein may be produced by cultivating the mutant strain with the desired heterologous protein gene incorporated downstream from the mutant AOX2 promoter. A plasmid carrying the mutant AOX2 promoter is also provided.

REFERENCES:
patent: 4882279 (1989-11-01), Cregg
patent: 4895800 (1990-01-01), Tschopp et al.
American Type Culture Collection, Catalogue of Yeasts, 18th Edition, 1990; Jong et al., EDS., ATCC, Maryland. pp. 45-50.
Koutz et al., (1989). Yeast 5(3):167-177.
Watson et al. (1983) in Recombinant DNA: A Short Course W. H. Freeman and Co. N.Y. p. 86.
Silhavy et al. 1984, In Experiments with Gene Fusions, Cold Spring Harbor Laboratory, N.Y. pp. 7-25.
Watson et al., EDS. "Molecular Biology of the Gene", Benjamin Kumminas Publishing Co., Inc., California., 1987, pp. 368-374 and 571-575.

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