Multiplex PCR primer set for human glucokinase gene...

Details Multiplex PCR primer set for human glucokinase gene... Multiplex PCR primer set for human glucokinase gene...

2006-06-06

2006-06-06

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C536S024330, C536S023100, C536S024300, C536S024310, C536S024320, C435S091100, C435S006120

Reexamination Certificate

active

07056705

ABSTRACT:
The present invention provides a primer pooi for amplifying a target sequence of a human glucokinase gene including at least one set of primers or variant primers thereof, each set of primers being identified by two consecutive SEQ ID NOS, the SEQ ID NOS starting at SEQ ID NO:3 and terminating at SEQ ID NO:24 according to the present invention. A target sequence such as the human glucokinase gene may be amplified with a high specificity, a high speed, a high sensitivity and a low cost through a multiplex PCR to detect a maturity-onset of diabetes of the young (MODY) 2 associated gene.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5541060 (1996-07-01), Bell et al.
patent: 5582989 (1996-12-01), Caskey et al.
patent: 6468744 (2002-10-01), Cronin et al.
patent: 0682120 (1995-11-01), None
patent: WO 9321343 (1993-10-01), None
Boehringer Mannheim, Critical Factors for Successful PCR. Boehringer Mannheim PCR Applications Manual, Boehringer Mannheim GmbH Biochemica, Germany, 1995.
Henegariu et al. Multiplex PCR: Critical parameters and Step-by-step protocol. Biotechniques, vol. 23, pp. 504-511, Sep. 1997.
Saiki et al.,Primer-Directed Enzymatic Amplification of DNA With a Thermostable DNA Polymerase, Science, vol. 239, Jan. 29, 1988, pp. 487-491.
Boutin, et al. “Routine Mutation Screening of HNF-1α and GCK genes in MODY Diagnosis: How Effective are the Techniques of DHPLC and Direct Sequencing used in Combination?”Diabetologia44:775-778 (2001).
European Search Report corresponding to EP 02024499. Mailed Nov. 10, 2003.
Henegariu, et al. “Multiplex PCR: Critical Parameters and Step-by-Step Protocol”BioTechniques23:504-511 (Sep. 1997).
Lehto, et al. “High Frequency of Mutations in MODY and Mitochondrial Genes in Scandinavian Patients with Familial Early-Onset Diabetes”Diabetologia42:1131-1137 (1999).

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