Multilayered microfluidic DNA analysis system and method

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

Reexamination Certificate

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C435S091100, C435S091200, C422S186290

Reexamination Certificate

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06984516

ABSTRACT:
A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.

REFERENCES:
patent: 5587128 (1996-12-01), Wilding et al.
patent: 5643789 (1997-07-01), Ducheyne et al.
patent: 6544734 (2003-04-01), Briscoe et al.

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