Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-06-02
2000-05-09
Allen, Marianne P.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435325, 4352523, C12N 1500
Patent
active
060602732
ABSTRACT:
Multicistronic expression units allow the equimolar expression of the genes located in the corresponding cistrons. These expression units are particularly suitable for the recombinant production of proteins composed of two or more polypeptide subunits.
REFERENCES:
patent: 5665567 (1997-09-01), Eichner et al.
Ostman et al., "Synthesis and Assembly of a Functionally Active recombinant Platelet-Derived Growth Factor AB Heterodimer", J. Biol. Chem. vol. 263, No. 31, Nov. 5, 1988, pp. 16202-16208.
Hart et al., "Purification of PDGF-AB and PDGF-BB from Human Platelet Extracts and Identification of all Three PDGF Dimers in Human Platelets", Biochemistry, vol. 29, No. 1, Jan. 9, 1990, pp. 166-172.
Falcone et al., "Both the 5' Untranslated region and the Sequences Surrounding the Start Site Contribute to Efficient Initiation of Translation in Vitro", Mol. Cell. Biol. vol. 11, No. 5, May 1991, p. 2662-2663.
Hammacher et al., "A Major Part of Platelet-Derived Growth Factor Purified from Human Platelets is a Heterodimer of one A and one B Chain", J. Biol. Chem. vol. 263, No. 31, Nov. 5, 1988, pp. 16493-16498.
Jackson et al., "The Novel Mechanism of Initiation of Picornavirus RNA Translation", Trends in Biochemical Science vol. 15, No. 12, Dec. 1990, pp. 477-483.
Kaufman et al., "Improved Vectors for Stable Expression of Foreign Genes in Mammalian Cells by Use of The Untranslated Leader Sequence from EMC Virus", Nucl. Acid Res. vol. 19, No. 16, Aug. 25, 1991, pp. 4485-4490.
Gossen et al., "Tight control of gene expression in mammalian cells by tetracycline-responsive promoters", Proc. Natl. Acad. Sci. USA 89:5547-5551 (1992).
Ptashne, Mark, "How eucaryotic transcriptional activators work", Nature 335:683-689 (1988).
Lieber et al, "High level gene expression in mammalian cells by a nuclear T7-phage RNA polymerase", Nucleic Acids Research 17(21):8485-8493 (1989).
Swanson et al, Production of Functional Human Hemoglobin in Transgenic Swine, Bio/Technology 10: 557 (1992).
Iizuka et al, Construction of Less Neurovirulent Polioviruses by Introducing Deletions into the 5' Noncoding Sequence of the Genome, J. Virology 63: 5354 (1989).
Kuhn et al, Functional Analysis of the Internal Translation Initiation Site of Foot-and-Mouth Disease Virus, J. Virology 64: 4625 (1990).
Beck et al, Structure of the FMDV Translation Initiation Site of the Structural proteins, Nucleic Acids Research, 11 # 22 (1983).
Jang et al, Cap-Independent Translation of encephalomyocarditis virus RNA: Structural Elements of the Internal Ribosomal Entry Site and Involvement of a cellular 57-kDRNA-binding Protein, Genes & Development 4:1560 (1990).
Dirks & Hauser, in Animal Cell Technology: Products for Today and Prospects for Tomorrow eds Spier et al (Butterworth pub.) Equimolar Expression of Two Protein Chains in Recombinant Mammalian Cells pp 610-617.
Jang et al, A Segment of the 5' Nontranslated Region of Encephalomyocarditis Virus RNA Directs Internal Entry of Ribosomes During In Vitro Translations, J. Virology 62: 2636 (1988).
Dirks et al, Bicistronic Transcription Units for Gene Expression in Mammalian Cells. Gene 128: 247 (1993).
Shipley et al, Differential Effects of Epidermal Growth Factor, Transforming Growth Factor, and Insulin on DNA and Protein Synthesis and Morphology in Serum Free Cultures of AKR-2B Cells, Can. Res. 44: 710 (1984).
Macejak et al, Internal Initiation of Translation Mediated by the 5' leader of a cellular mRNA, Nature 353: 90 (1991).
Jackson et al, A Novel mechanism of Initiation of Picornavirus RNA Translation, TIBS Dec. 1990 p. 477-483.
Wilmut et al, Nature 385:810 (1997), Viable Offspring derived from fetal and adult mammalian cells.
Klon-Schaf mit menschlichen Genen geboren Jul. 25, 1997.
Palmiter et al. Ann Rev. Genetics 20: 465 (1986) Germ Line Transformation of Mice.
Achterberg Volker
Dirks Wilhelm
Dorschner Albrecht
Eichner Wolfram
Hauser Hansjorg
Allen Marianne P.
Beiersdorf AG
Gesellschaft fur Biotechnologische Forschung mbH
LandOfFree
Multicistronic expression units and their use does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Multicistronic expression units and their use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Multicistronic expression units and their use will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1063835