Monoclonal antibodies to the light chain region of human factor

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530380, 530382, 530383, 530388, 530413, 530415, 530417, 43524027, 435 5, 435 7, 424 858, A61K 39395, C07K 1528

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049636570

ABSTRACT:
Novel cell lines produce monoclonal antibody to the light chain region of human factor XII. Three such cell lines, ATCC #HB-9703 through ATCC #HB-9704, are formed by fusing SP2/0-Ag14 myeloma cells with spleen cells from BALB/c AnSkh mice immunized with human factor XIIf. Biochemical and therapeutic uses of the monoclonal antibodies are provided.

REFERENCES:
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Clarke et al., J. Biol. Chem., 264(19), 11497-502, (7/89).
Campbell, Monoclonal Antibody Technology, chapters 3,4 and 6-10 as supplied, (Elsevier, N.Y.,) 1985.
Saito et al., "Production and Characterization of a Murine Monoclonal Antibody Against a Heavy Chain of Hageman Factor (Factor XII)", Blood 65:1263-1268 (1985).
Small et al., "A Monoclonal Antibody That Inhibits Activation of Human Hageman Factor (Factor XII)" Blood, 65:202-210 (1985).
Laemmle et al., "Enhanced Specificity of Immunoblotting Using Radiolabeled Antigen Overlay: Studies of Bood Coagulation Factor XII and Prekallikrein in Plasma" Anal. Biochem. 156:118-125 (1986).
Laemmle etal., "Quantitative Immunoblotting Assay of Blood Coagulation Factor XII" Thromb. Res. 41:747-759 (1986).
Pixley et al., "A Monoclonal Antibody Recognizing an Icosapeptide Sequence in the Heavy Chain of Human Factor XII Inhibits Surface-Catalyzed Activation" J. Biol. Chem. 262:10140-10145 (1987).
Sugihara et al., "Application of a Monoclonal Antibody Against Factor XII (Hageman Factor); Enzyme-Linked Immunosorbent Assay (ELISA) for Factor XII Antigen", Acta Haematologica Japonica 50:691-5 (May 1987).

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