Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1984-11-29
1988-06-21
Moskowitz, Margaret
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 7, 435 68, 435 70, 4351722, 935104, 935110, 436548, 530387, C12N 500, C07K 1504
Patent
active
047525835
ABSTRACT:
The present invention provides monoclonal antibodies specific to and distinguish between hemoglobin S and hemoglobin A and methods for their production and use. These antibodies are capable of distinguishing between two hemoglobin types which differ from each other by only a single amino acid residue. The antibodies produced according to the present method are useful as immunofluorescent markers to enumerate circulating red blood cells which have the property of altered expression of the hemoglobin gene due to somatic mutation in stem cells. Such a measurement is contemplated as an assay for in vivo cellular somatic mutations in humans. Since the monoclonal antibodies produced in accordance with the instant invention exhibit a high degree of specificity to and greater affinity for hemoglobin S, they are suitable for labeling human red blood cells for flow cytometric detection of hemoglobin genotype.
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Stamatoyannopoulos, G. et al., Blood 61:(3):530-539 (1983).
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Jensen, R. H. et al., UCRL-88226, Lawrence Livermore National Laboratory, Jan. 23, 1983.
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Bigbee, W. L. et al., Individual Susceptibility to Genotoxic Agents in the Human Population, de Serres, F. et al., eds., Plenum Publishing Co. (1984), pp. 249-266.
Jensen, R. H. et al., Biological Dosimetry, Eisert, W. G. et al. eds., Springer-Verlag, Berlin (1984), pp. 161-170.
Bigbee William L.
Branscomb Elbert W.
Grabske Robert J.
Jensen Ronald H.
Stanker Larry H.
Carnahan L. E.
Hightower Judson R.
Moskowitz Margaret
Rajendar Shyamala T.
The United States of America as represented by the United States
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