Monoclonal antibodies to factor VIIIC

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4351722, 435 68, 436548, 935 89, 935 95, 935102, 935103, 935104, 935105, 935106, 935108, 935110, 424 85, A61K 39395, G01N 33597

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047161175

ABSTRACT:
Hybridomas producing monoclonal antibodies specific for polypeptide fragments derived from human Factor VIIIC are provided. Class I hybridomas produce monoclonal antibodies reactive with a 80/77 kd doublet fragment or with both the 80/77 kd doublet and a 240 kd polypeptide. Class III hybridomas produce monoclonal antibodies reactive with the 240 kd polypeptide as well as a 92.5 kd fragment and its precursors. Class III antibodies show additional reactivity with a 40 kd thrombin digestion product. The monoclonal antibodies are useful for the separation of Factor VIIIC and its constituent polypeptides, as well as for the immunoassay of Factor VIIIC in biological samples.

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