Molecular topological fractionation of macromolecules

Details Molecular topological fractionation of macromolecules Molecular topological fractionation of macromolecules

2003-05-14

2008-08-26

Kornakov, Mikhail (Department: 4151)

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Means for analyzing liquid or solid sample

C702S023000, C702S030000, C210S656000

Reexamination Certificate

active

07416698

ABSTRACT:
A process for characterizing a sample comprising a population of linear macromolecules of interest (104) and a population of long chain branched macromolecules of interest (103), the process including four steps. The first step is to provide a flow through separating medium (100) and a liquid eluant (101) in which the macromolecules of interest dissolve, the separating medium defining flow through channels (102), the eluant flow rate and the average diameter of the channels being in a range so that the linear macromolecules of interest elute before the long chain branched macromolecules of interest (105) (106). The second step is to introduce a sample into the liquid eluant. The third step is to flow the liquid eluant under pressure through the channels (102) of the separating medium (100). The fourth step is to differentiate the linear macromolecules of interest (104) from the long chain branched macromolecules of interest (103) based on their successive elution volumes established in the third step such as by determining the refractive index of the successive elution volumes or by subjecting the successive elution volumes to size exclusion chromatography.

REFERENCES:
patent: 4112032 (1978-09-01), Blaszyk et al.
patent: 4775943 (1988-10-01), Chamberlin et al.
patent: 6294388 (2001-09-01), Petro
patent: WO 99/42489 (1999-08-01), None
H. Yun, S.V. Olesik and E.H. Marti, J. Microcolumn Separations 11:53-61, 1999.
K. Cabrera et al., J. High Resol. Chromatogr., 2000, 23, (1) 93-99.
Q. Ching Wang et al., Anal. Chem., 1993, 65, 2243-2248.
Frantisek Svec et al., Ind. Eng. Chem. Res., 1999, 38, 34-48.
Markus Gerle et al., Macromoleules, 1999, 32, 2629-2637.
W. W. Graessley et al., Macromolecules, 1976, 9 (1), 127-141.
M. De Pooter et al., J. of Applied Polymer Science, 1991, 42, 399-408.
M. Muthukumar et al., Macromolecules, 1989, 22, 1937-1941.
Norio Ishizuka et al., J. of Chromatography A, 1998, 797, 133-137.
Fred E. Regnier, J. High Resol. Chromatogr. 2000, 23, (1) 19-26.
Miroslav Petro et al., J. of Chromatography A, 1996, 752, 59-66.
Miroslav Janco et al., J. of Polymer Science: Part A: Polymer Chem., 38, 2000, 2767-78.
Donna J. Frater et al., J. of Polymer Science: Part B: polymer Physics, 35, 1997, 141-151.
S. Podzimek et al., J. of Applied Polmer Science, 81, 1588-1594 (2001).
Jiro Kumaki et al., J. Am. Chem. Soc., 1996, 118, 3321-3322.
Svetlana Prokhorova et al., Macromolecules, 1999, 32, 2653-2660.
Timothy von Werne et al., J. Am. Chem. Soc., 1999, 121, 7409-7410.
Isabelle Gusev et al., J. of Chroma. A. 855 (1999, 273-290.
E.E. Drott et al., J. of Poly. Sci.: Part a-2, 8, 1373-1385, 1970.
Gerrit Stegeman et al., J. of Chrom. A, 657, 1993, 283-303.
Hamish Small, J. of Colloid and Interface Science, vol. 48, No. 1, 1974.
T.A.J. Duke et al., Physical Review Letters, vol. 80, No. 7, 1552-1555, 1998.
David L. Smisek et al., Science, vol. 248, 1221-1223, 1990.
J. Brandrup and E.H. Immergut, Polymer Handbook, 1989, John Wiley & Sons.
J. Janca, Field-Flow Fractionation —Analysis of Macromolecules and Particles, 1988, Marcel Dekker.
J. C. Randall, NMR and Macromolecules, ACS, Washington, D.C., 1984, Chapter 9.
C. W. Macosko, Rheology, Principles, Measurements, and Applications, Wiley-VCH, New York, 1994, p. 505.
J. Calvin Giddings, Unified Separation Science, Wiley & Sons, Inc., New York, 1991.
R. K. Iier, The Chemistry of Silica, Wiley & Sons, Inc, New York, 1979, pp. 539-544.
W.W. Yau et al., Modern Size-Exclusion Liquid Chromatography, 1979, Wiley & Sons, Inc.
Hahnfeld, et al.,Applications of Anionic Polymerization Research, Quick, Ed., ACS Symposium Series 696, pp. 167-184, 1996.
E. Heftmann Ed.,Chromatography Fundamentals and applications of chromatographic and electrophoretic methods, vol. 22A, 1983, pp. A38-39.

Affiliated with

Also associated with

Rating

Molecular topological fractionation of macromolecules does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Molecular topological fractionation of macromolecules, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Molecular topological fractionation of macromolecules will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4005971