Midecamycin hyper producing strain

Details Midecamycin hyper producing strain Midecamycin hyper producing strain

2006-03-29

2011-10-04

Leavitt, Maria (Department: 1633)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S183000, C435S252350, C536S023200, C536S023700, C536S023100

Reexamination Certificate

active

08030052

ABSTRACT:
A midecamycin hyper producing strain having improved productivity of midecamycin which is a member of macrolide antibiotics, and a method for producing midecamycins using the strain are provided. The midecamycin producing actinomycetes comprises a midecamycin biosynthesis gene or a homologue thereof, wherein at least one module in a polyketide synthase gene of a midecamycin biosynthesis gene or partial sequences of the at least one module, is substituted so as to encode the corresponding amino acid sequences of the other module.

REFERENCES:
patent: 3761588 (1973-09-01), Tsuruoka et al.
patent: 7070980 (2006-07-01), Midoh et al.
patent: 2004/0091975 (2004-05-01), Midoh et al.
patent: 2004-049100 (2004-02-01), None
Guo et al., Protein tolerance to random amino acid change. Proc Natl Acad Sci U S A. Jun. 22, 2004;101(25):9205-10. Epub Jun. 14, 2004.
Kimchi-Sarfaty Cet al., A “silent” polymorphism in the MDR1 gene changes substrate specificity. Science. Jan. 26, 2007;315(5811):525-8.
Wiley et al., 1990, Biochemistry, pp. 126-129.
Stefano Donadio, et al., “Modular Organization of Genes Required for Complex Polyketide Biosynthesis,” Research Articles, May 3, 1991, pp. 675-679.
Torsten Schwecke, et al., “The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin,” Proc. Natl. Acad. Sci. USA, vol. 92, pp. 7839-7843, Aug. 1995, Biochemistry.
Axel A. Brakhage, “Molecular Regulation of β-Lactam Biosynthesis in Filamentous Fungi,” Microbiology and Molecular Biology Reviews, Sep. 1998, pp. 547-585, vol. 62, No. 3.
Hans Weiher, et al., “Segment-specific mutagenesis: Extensive mutagenesis of alacpromoter/operator element,” Proc. Natl. Acad. Sci. USA, vol. 79, pp. 1408-1412, Mar. 1982, Biochemistry.
Thomas Kunkel, “Rapid and efficient site-specific mutagenesis without phenotypic selection,” Proc. Natl. Acad. Sci. USA, vol. 82, pp. 488-492, Jan. 1985, Genetics.
CR Hutchinson et al., “Genetic engineering of doxorubicin production inStreptomyces peucetius: a review,” Journal of Industrial Microbiology & Biotechnology (1999) 23, 647-652.
Omoto et al.; “Modifications of a Macrolide Antibiotic Midecamycin (SF-837)”; The Journal of Antibiotics; May 1976; pp. 536-548; vol. 29, No. 5.
Yoshida et al.; “Bacteriological Evaluation of Midecamycin Acetate and its Metabolites”; The Japanese Journal of Antibiotics; Jun. 1982, pp. 1462-1475; vol. 35, No. 6.
MacNeil et al.; Complex organization of theStreptomyces avermitilisgenes encoding the avermectin polyketide synthase; Gene; 1992; pp. 119-125; vol. 115.
Summers et al.; “Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster ofSaccharopolyspora erythraeathat are involved in L-mycarose and D-desosamine production”; Microbiology; 1997; pp. 3251-3262; vol. 143.
Gaisser et al.; “Analysis of seven genes from the eryAl-eryK region of the erythromycin biosynthetic gene cluster inSaccharopolyspora erythraea”; Mol. Gen. Genet.; 1997; pp. 239-251; vol. 256.
L. A. Merson-Davies and E. Cundliffe; “Analysis of five tylosin biosynthetic genes from the tylIBA region of theStreptomyces fradiaegenome”; Molecular Microbiology; 1994; pp. 349-355; vol. 13, No. 2.
J. Kennedy and G. Turner; “δ(L-x-Aminoadipyl)-L-cysteinyl-D-valine synthetase is a rate limiting enzyme for penicillin production inAspergillus nidulans”; Mol. Gen. Genet.; 1996; pp. 189-197; vol. 253.
R. H. Baltz; “Molecular Genetic Approaches to Yield Improvement in Actinomycetes”; Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN; pp. 49-62.
Bierman et al.; “Plasmid cloning vectors for the conjugal transfer of DNA fromEscherichia colito Streptomyces spp.”; Gene; 1992; pp. 43-49; vol. 116.
H. Shiraishi and Y. Shimura; “A rapid and efficient method for targeted random mutagenesis”; Gene; 1988; pp. 313-319; vol. 64.
Ito et al.; “A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction”; Gene; 1991; pp. 67-70; vol. 102.
Hashimoto-Gotoh et al.; “An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenisis”; Gene; 1995; pp. 271-275; vol. 152.
T.-J. Shen et al.; “A marker-coupled method for site-directed mutagenesis”; Gene; 1991; pp. 73-77; vol. 103.
Notification of First Office Action issued in counterpart Chinese Application No. 200610073849.7 dated Jan. 22, 2010.
Japanese Office Action issued on Sep. 14, 2010 in the corresponding Japanese Patent Application No. 2005-101836.

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