Microfluidic analysis system

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Calorimeter

Reexamination Certificate

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Reexamination Certificate

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07622076

ABSTRACT:
A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

REFERENCES:
patent: 5270183 (1993-12-01), Corbett et al.
patent: 6294063 (2001-09-01), Becker et al.
patent: 6306590 (2001-10-01), Mehta et al.
patent: 6670153 (2003-12-01), Stern
patent: 2003/0170698 (2003-09-01), Gascoyne et al.
patent: WO99/41015 (1999-08-01), None
patent: WO03/016558 (2003-02-01), None
Yang J.; Huang, Y.; Wang, X.-B.; Becker, F. F.; Gascoyne, R.C. “Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation.” Anal. Chem. 1999, 71, pp. 91-918.

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