Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2007-11-13
2007-11-13
Beisner, William H. (Department: 1744)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C435S288700, C435S293100, C422S082000, C436S053000, C436S063000, C204S604000
Reexamination Certificate
active
09953103
ABSTRACT:
A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.
REFERENCES:
patent: 2656508 (1953-10-01), Coulter
patent: 3570515 (1971-03-01), Kinner
patent: 3747628 (1973-07-01), Holster et al.
patent: 4046159 (1977-09-01), Pegourie
patent: 4119368 (1978-10-01), Yamakazi
patent: 4153855 (1979-05-01), Feingold
patent: 4245673 (1981-01-01), Bouteille et al.
patent: 4373527 (1983-02-01), Fischell
patent: 4399219 (1983-08-01), Weaver
patent: 4434704 (1984-03-01), Surjaatmadja
patent: 4575681 (1986-03-01), Grosso et al.
patent: 4585209 (1986-04-01), Aine et al.
patent: 4662710 (1987-05-01), ten Berge
patent: 4898582 (1990-02-01), Faste
patent: 4992312 (1991-02-01), Frisch
patent: 5085562 (1992-02-01), Van Lintel
patent: 5088515 (1992-02-01), Kamen
patent: 5096388 (1992-03-01), Weinberg
patent: 5126115 (1992-06-01), Fujita et al.
patent: 5164558 (1992-11-01), Huff et al.
patent: 5171132 (1992-12-01), Miyazaki
patent: 5224843 (1993-07-01), Van Lintel
patent: 5259737 (1993-11-01), Kamisuki et al.
patent: 5265327 (1993-11-01), Faris et al.
patent: 5290240 (1994-03-01), Horres, Jr.
patent: 5336062 (1994-08-01), Richter
patent: 5346372 (1994-09-01), Naruse et al.
patent: 5375979 (1994-12-01), Trah
patent: 5376252 (1994-12-01), Ekstrom
patent: 5400741 (1995-03-01), DeTitta et al.
patent: 5417235 (1995-05-01), Wise et al.
patent: 5423287 (1995-06-01), Usami et al.
patent: 5452878 (1995-09-01), Gravesen et al.
patent: 5454472 (1995-10-01), Benecke et al.
patent: 5529465 (1996-06-01), Zengerle et al.
patent: 5574893 (1996-11-01), Southgate et al.
patent: 5593130 (1997-01-01), Hansson et al.
patent: 5642015 (1997-06-01), Whitehead et al.
patent: 5656155 (1997-08-01), Norcross et al.
patent: 5659171 (1997-08-01), Young et al.
patent: 5660370 (1997-08-01), Webster
patent: 5661222 (1997-08-01), Hare
patent: 5665070 (1997-09-01), McPhee
patent: 5681024 (1997-10-01), Lisec et al.
patent: 5705018 (1998-01-01), Hartley
patent: 5759014 (1998-06-01), Van Lintel
patent: 5775371 (1998-07-01), Pan et al.
patent: 5788468 (1998-08-01), Dewa et al.
patent: 5836750 (1998-11-01), Cabuz
patent: 5842787 (1998-12-01), Kopf-Sill et al.
patent: 5854684 (1998-12-01), Stabile et al.
patent: 5875817 (1999-03-01), Carter
patent: 5876187 (1999-03-01), Forster et al.
patent: 5932799 (1999-08-01), Moles
patent: 5942443 (1999-08-01), Parce et al.
patent: 5997961 (1999-12-01), Feng et al.
patent: 6007309 (1999-12-01), Hartley
patent: 6043080 (2000-03-01), Lipshutz et al.
patent: 6123769 (2000-09-01), Sanjoh
patent: 6155282 (2000-12-01), Zachary et al.
patent: 6174365 (2001-01-01), Sanjoh
patent: 6221654 (2001-04-01), Quake et al.
patent: 6246330 (2001-06-01), Nielsen
patent: 6281254 (2001-08-01), Nakajima et al.
patent: 6296673 (2001-10-01), Santarsiero et al.
patent: 6329209 (2001-12-01), Wagner et al.
patent: 6345502 (2002-02-01), Tai et al.
patent: 6358387 (2002-03-01), Kopf-Sill et al.
patent: 6375871 (2002-04-01), Bentsen et al.
patent: 6376971 (2002-04-01), Petrine et al.
patent: 6409832 (2002-06-01), Weigl et al.
patent: 6488832 (2002-12-01), Heller
patent: 6488872 (2002-12-01), Beebe et al.
patent: 6520936 (2003-02-01), Mann
patent: 6540895 (2003-04-01), Spence et al.
patent: 6541071 (2003-04-01), Bookbinder et al.
patent: 6667124 (2003-12-01), Suenaga et al.
patent: 6689473 (2004-02-01), Guire et al.
patent: 6713327 (2004-03-01), Leedy
patent: 6716378 (2004-04-01), Yang et al.
patent: 6765279 (2004-07-01), Leedy
patent: 6767706 (2004-07-01), Quake et al.
patent: 6829753 (2004-12-01), Lee et al.
patent: 6847153 (2005-01-01), Balizer
patent: 6866785 (2005-03-01), Zare et al.
patent: 6884346 (2005-04-01), Zare et al.
patent: 2001/0027745 (2001-10-01), Weigl et al.
patent: 2001/0048637 (2001-12-01), Weigl et al.
patent: 2002/0005354 (2002-01-01), Spence et al.
patent: 2002/0014673 (2002-02-01), Leedy
patent: 2002/0037499 (2002-03-01), Quake et al.
patent: 2002/0045297 (2002-04-01), Leedy
patent: 2002/0108096 (2002-08-01), Lee et al.
patent: 2003/0080442 (2003-05-01), Unger
patent: 2004/0248167 (2004-12-01), Quake et al.
patent: 2005/0065735 (2005-03-01), Lee et al.
patent: 0 592 094 (1994-04-01), None
patent: 0 703 364 (1996-03-01), None
patent: 0 706 004 (1996-04-01), None
patent: 0 779 436 (1997-06-01), None
patent: 0 829 360 (1998-03-01), None
patent: 0 845 603 (1998-06-01), None
patent: 0 999 055 (2000-05-01), None
patent: 1065378 (2001-01-01), None
patent: 2097692 (1982-11-01), None
patent: 2 155 152 (1985-09-01), None
patent: 2 308 460 (1997-06-01), None
patent: WO 98/07069 (1998-02-01), None
patent: WO98/52691 (1998-11-01), None
patent: WO 99/00655 (1999-01-01), None
patent: WO 99/04361 (1999-01-01), None
patent: WO 99/17093 (1999-04-01), None
patent: WO 99/52633 (1999-10-01), None
patent: WO 99/61888 (1999-12-01), None
patent: WO 00/00678 (2000-01-01), None
patent: WO 00/43748 (2000-07-01), None
patent: WO 00/60345 (2000-10-01), None
patent: WO 01/06529 (2001-01-01), None
patent: WO 01/06575 (2001-01-01), None
patent: WO 01/09595 (2001-02-01), None
patent: WO 01/09595 (2001-02-01), None
patent: WO 02/082047 (2002-10-01), None
Angell et al., “Silicon Micromechanical Devices”, Scientific American 248:44-55 (1983).
Ashkin A., Dziedzic J.M., “Optical Trapping and Manipulation of Viruses and Bacteria”, Science 235, 1517-1520 (1987).
Ashkin A., Dziedzic J.M., “Optical trapping and manipulation of single cells using infrared laser beams”, Nature 330, 769-771 (1987).
Buican T.N., Smyth M.J., Verissamn H.A., “Automated single-cell manipulation and sorting by light trapping”, Applied Optics 26, 5311-5316 (1987).
Chow H.P., Schere A., Spence C., Quake S. R., “A microfabricated device for sizing and sorting DNA molecules”, Proc. Natl. Aca. Sci. USA 96: 11-13 (1998).
Crosland-Taylor P.J., “A Device for Counting Small Particles suspended in a Fluid through a Tube”, Nature (London) 171, 37-38 (1953).
Fiedler S., et al. “Dielectrophoretic Sorting of Particles and Cells in a Microsystem”, Analytical Chemistry 70, 1909-1915 (1998).
Fulwyler M.J., “Electronic separation of biological cells by volume”, Science 150, 910-911 (1965).
Harrison et al., Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip, Science, 261:895-897 (1993).
Kamensky L.A., Melamed M.R., Derman H., “Spectrophotometer: New Instrument for Ultrarapid Cell Analysis”, Science 150, 630-631 (1965).
Kopp M.U. et al., “Chemical Amplification: Continuous-Flow PCR on a Chip”, Science, 280: 1046-1048 (1998).
Li P.H., Harrison D.J., “Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects”, Analytical Chemistry 69, 1564-1568 (1997).
Manz et al., “Micromachining of monocrystalline silicon and glass for chemical analysis systems”, Trends in Analytical Chemistry 10: 144-149 (1991).
Mason, T.J. and Bibette, J. “Shear Rupturing of Droplets in Complex Fluids”, Langmuir, 13, 4600-4613 (1997).
Moldavan A., “Scientific Apparatus and
Quake Stephen R.
Thorsen Todd
Apple Ted
Beisner William H.
California Institute of Technology
Collier Steven W.
Townsend and Townsend / and Crew LLP
LandOfFree
Microfabricated crossflow devices and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Microfabricated crossflow devices and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Microfabricated crossflow devices and methods will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3823541