Microbial inhibition test kit and method

Details Microbial inhibition test kit and method Microbial inhibition test kit and method

1991-11-12

1994-10-11

Witytshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 34, 43525231, 4352525, 435287, 435291, 435300, C12Q 100, C12Q 102, C12Q 104, C12M 100

Patent

active

053546636

ABSTRACT:
A test kit and method for the detection of antimicrobial drugs at low concentrations in a sample in a test container, such as milk, which includes a Bacillus stearothermophilus (BST) tablet and a medium tablet with nutrients to stress the BST and, optionally, a pH indicator. The method comprises preheating a test sample to destroy natural inhibitors, cooling the sample, adding a BST tablet, rapidly heating to effect synchronization of BST germination, adding a medium tablet, incubating and detecting the presence or absence of the antimicrobial drug. The test apparatus includes optionally a test apparatus for the controlled, sequenced heating and cooling of the test sample.

REFERENCES:
patent: 4303752 (1981-12-01), Kolehmainen et al.
patent: 4704360 (1987-11-01), Shoham et al.
Cooper, T. "The Tools of Biochemistry" pp. 366-377 John Wiley & Sons, 1977.
Hartmanis, et al. Appl Microbiol Biotechnol pp. 369-371 (1986).

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