Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-06-29
1996-12-10
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 254, 546109, C12Q 168
Patent
active
055829848
ABSTRACT:
Phenanthridium compounds are provided which are comprised of intercalators and substituted intercalator segments having a functionalized chain or chains which provide a high affinity for binding to the DNA molecule and show reduced self-quenching, while providing superior transport kinetics. The compounds have been found to provide enhanced fluorescence when bound to a DNA molecule within a fluorescent flow cytometry environment which is about eight to ten times brighter in fluorescence than "bis" structure intercalators and other known intercalators utilized in flow cytometry environment, said enhancement of fluorescence allows a drastic reduction in the amount of intercalator needed for applications of similar sensitivity than those using known intercalators. Substantial improvements in material use efficiency, accuracy and dependability of various DNA studies are direct results of the inventive intercalator compounds, said compounds having use in known DNA intercalator applications inclusive of flow cytometry, gel electrophoresis, DNA detection, immunoassay for DNA, fluorescence in situ hybridization assays, DNA sequencing application, therapeutic application and conversion staining methodologies.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Neidle, Basic Life Sci., 38(Mechanisms DNA Damage Repair), pp. 257-264, Ed. by Simic et al., Pienum Pres, N.Y. 1986.
Roques et al., C. R. Hebd. S eances Acad. Sci., Ser. D, 283(11), 1365-7, 1976.
Bieniarz Christopher
Henrard Denis R.
Huff Jeffrey B.
Abbott Laboratories
Houtteman Scott W.
Jones W. Gary
Weinstein David L.
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