Methods of screening for agonists and antagonists for human CCR7

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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435 71, 435 72, 435 721, 435 691, 435 695, G01N 33566, G01N 3353, G01N 33567, C12P 2106, C12P 2102

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active

061534412

ABSTRACT:
Disclosed are methods for discovering agonists and antagonists of the interaction between a secreted human protein, chemokine CK.beta.-9, and its cellular receptor, human CCR7, which may have utility in the treatment of several human diseases, including, but not limited to: allergic disorders, autoimmune diseases, ischemia/reperfusion injury, development of atherosclerotic plaques, cancer (including mobilization of hematopoietic stem cells for use in chemotherapy or mycloprotection during chemotherapy), chronic inflammatory disorders, chronic rejection of transplanted organs or tissue grafts, chronic myelogenous leukemia, and infection by HIV and other pathogens.

REFERENCES:
patent: 5605817 (1997-02-01), Coleman et al.
Hedrick, et al., "Identification and Characterization of a Novel .beta. Chemokine Containing Six Conserved Cysteines," The Journal of Immunology, 159: pp. 1589-1593 (1997).
Hromas, et al., "Isolation and Characterization of Exodus-2, a Novel C-C Chemokine with a Unique 37-Amino Acid Carboxyl-Terminal Extension," The Journal of Immunology, 159: pp. 2554-2558 (1997).
Nagira, et al., "Molecular Cloning of a Novel Human CC Chemokine Secondary Lymphoid-Tissue Chemokine That Is a Potent Chemoattractant for Lymphocytes and Mapped to Chromosome 9p13," The American Society for Biochemistry and Molecular Biology, Inc., 272 (Nov. 31, Issue of Aug. 1, 1997): pp. 19518-19524 (1997).
Li, T. et al, Proc Natl Acad Sci USA 92:8(3551-5), Apr. 1995.

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