Methods of producing recombinant insulin from novel fused...

Details Methods of producing recombinant insulin from novel fused... Methods of producing recombinant insulin from novel fused...

2005-01-11

2005-01-11

Carlson, Karen Cochrane (Department: 1653)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

Reexamination Certificate

active

06841361

ABSTRACT:
This invention relates to a DNA encoding a fusion protein of formula (I)in-line-formulae description="In-line Formulae" end="lead"?[Y]-[X1]-[B-chain]-[X2]-[Linker]-[X3]-[A-chain]  (I)in-line-formulae description="In-line Formulae" end="tail"?whereinY is a leader peptide sequence for the expression and secretion of the protein, comprising at least one amino acid residue;X1 is an amino acid sequence which is cleavable enzymatically or chemically;B-chain is the amino acid sequence of insulin B chain;X2 is an amino acid sequence which is cleavable enzymatically;Linker is a linker sequence comprising at least one amino acid residue;X3 is an amino acid sequence which is cleavable enzymatically; andA- chain is the amino acid sequence of insulin A chain,and to a method for producing insulin using an expression system containing the DNA in high efficiency and high yield.

REFERENCES:
patent: 4431740 (1984-02-01), Bell et al.
patent: 4994380 (1991-02-01), Udaka et al.
patent: 5149716 (1992-09-01), Vertesy et al.
patent: 5378613 (1995-01-01), Belagaje
patent: 5426036 (1995-06-01), Koller et al.
patent: 5473049 (1995-12-01), Obermeier et al.
patent: 5663291 (1997-09-01), Obermeier et al.
patent: 5962267 (1999-10-01), Shin et al.
patent: 6001604 (1999-12-01), Hartman et al.
patent: 0055945 (1982-07-01), None
patent: 0531530 (1990-03-01), None
patent: 0704527 (1996-04-01), None
patent: A2 704527 (1996-04-01), None
patent: 2 072 680 (1981-10-01), None
patent: A11-341991 (1999-12-01), None
patent: 11-341991 (1999-12-01), None
patent: 9620724 (1996-07-01), None
patent: A 9632489 (1996-10-01), None
patent: WO9632489 (1996-10-01), None
patent: WO 9703089 (1997-01-01), None
Yamagata H, Adachi T, Tsuboi A, Takao M, Sasaki T, Tsukagoshi N, Udaka S. Cloning and characterization of the 5′ region o the cell wall protein gene operon in Bacillus brevis 47.J Bacteriol. Mar. 1987;169(3):1239-45.*
Nilsson et al., Journal of Biotechnology, 48 pp. 241-250 (1996).
Simonson et al., Human Gene Therapy, vol. 7, pp. 71-78 (Jan. 1, 1996).
Groskreutz et al., The Journal of Biological Chemistry, vol. 269, No. 8, pp. 6241-6245 (Feb. 25, 1994).
Udaka et al., Methods in Enzymology, vol. 217, pp. 23-33 (1993).
Miyauchi et al., Journal of Industrial Microbiology & Biotechnology, vol. 21, pp. 208-214 (1998).
Hideo Yamagata et al., “Cloning and Characterization of the 5′ Region of the Cell Wall Protein Gene Operon in Bacillus brevis 47”, Journal of Bacteriology, vol. 169(3) Mar. 1987, pp. 1239-1245.
Makoto Takao et al., “Production of swine pepsinogen by protein-producing Bacillus brevis carrying swine pepsinogen cDNA”, Appl. Microbiol. Biotechnol., vol. 30, 1989, pp. 75-80.
Hideo Yamagata et al., “Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor”, Proc. Natl. Acad. Sci. USA, vol. 86, May 1989, pp. 3589-3593.

Affiliated with

Also associated with

Rating

Methods of producing recombinant insulin from novel fused... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of producing recombinant insulin from novel fused..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of producing recombinant insulin from novel fused... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3383342