Methods of improving viability of cells at low temperature

Details Methods of improving viability of cells at low temperature Methods of improving viability of cells at low temperature

1998-05-08

2000-02-22

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Adaptation or attenuation of cells

435 4, 435 72, 435325, 435410, 435233, 435375, C12N 990

Patent

active

06027932&

ABSTRACT:
Methods are disclosed which employ cold-shock proteins, such as Trigger Factor, to confer cold-tolerance to cells and to enhance viability of cells at temperatures at which the cells would not normally remain viable. Proteins having peptidyl-proline isomerase activity are also disclosed as being useful to confer cold-tolerance to cells. Cells which overexpress cold-shock proteins are useful in cell-based expression systems which are intended to express proteins at low temperatures.

REFERENCES:
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patent: 5470971 (1995-11-01), Kondo et al.
patent: 5827685 (1998-10-01), Lindquist
Galat, 1993, "Peptidyl cis-trans-isomerases: immunophilins", Eur. J. Biochem., 216(3): 689-707.
Guthrie and Wickner, 1990, "Trigger factor depletion or overproduction causes defective cell division but does not block protein export", J. Bacteriol., 172(10): 5555-5526.
Jones, Van Bogelen and Neidhardt, 1987, "Induction of proteins in response to low temperature in Escherichia coli", J. Bacteriol., 169(5): 2092-2095.
Jones, Cashel, Glaser and Neidhardt, 1992, J. Bacteriol., "Function of a relaxed-like state following temperature downshifts in Escherichia coli", 174(12): 3903-3914.
Kandror, Sherman, Rhode and Goldberg, 1995, "Trigger factor is involved in GroEL-dependent protein degradation in Escherichia coli and promotes binding of GroEL to unfolded proteins", EMBO J., 14(23): 6021-6027.
Kandror, Sherman, Moerschall and Goldberg, 1997, "Trigger factor associates with GroEL in vivo and promotes its binding to certain polypeptides", J. Biol. Chem., 272(3): 1730-1734.
Ng, Ingraham and Mar, 1962, "Damage and derepression in Escherichia coli resulting from growth at low temperatures", J. Bacteriol., 84: 331-339.
Rudd, Sofia, Koonin, Plunkett, Lazar and Rouviere, 1995, "A new family of peptidyl-prolyl isomerases", Trends Biochem. Sci., 20(1): 12-14.
Van Bogelen and Neidhardt, 1990, "Ribosomes as sensors of heat and cold shock in Escherichia coli", Proc. Natl. Acad. Sci. U.S.A., 87(15): 5589-5593.

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