Methods of identifying compounds that modulate IL-4...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Reexamination Certificate

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10197962

ABSTRACT:
The present provides compounds capable of modulating IL-4 receptor-mediated IgE production, as well as IL-4 induced processes associated therewith, methods and kits for identifying such compounds that utilize a thioredoxin-like 32 kDa protein (TXNL) as a surrogate analyte and methods of using the compounds in a variety of in vitro, in vitro and ex vivo contexts.

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Chen X. et al., Crystallization and preliminary X-ray analysis of a Trx domain of human thioredoxin-like protein, ACTA Cryst. D57:1712-1714 (2001).
Lee K. et al., Purification, Molecular Cloning, and Characterization of TRP32, a Novel Thioredoxin-related Mammalian Protein of 32 kDa, The Journal of Biological Chemistry 273:30:19160-19166 (1998).
Miranda-Vizuete et al., Molecular Cloning and Expression of a cDNA Encoding a Human Thioredoxin-like Protein, Biochemical and Biophysical Research Communications 243:284-288 (1998).
Miranda-Vizuete et al., Identification of a Novel Thioredoxin-I Pseudogene on Human Chromosome 10, DNA Sequence 10(6):411-414 (2000).
Miranda-Vizuete et al., Genomic Structure and Chromosomal Localization of Human Thioredoxin-Like Protein Gene (txl) DNA Sequence 10(6): 419-424 (2000).
Miranda et al., 2000, Genomic Structure and Chromosomal Localization of Human Thioredoxin-Like Protein Gene (txl),DNA Sequence10(6):419-424.
Databases Unisave, “Thioredoxin-like protein (32kDa thoredoxin-like protein),” Accession No. 043396 (Jun. 6, 2002).

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